Intervertebral Disc Repair Using Adipose Tissue-Derived Stem and Regenerative Cells Experiments in a Canine Model
Intervertebral Disc Repair Using Adipose Tissue-Derived Stem and Regenerative Cells Experiments in a Canine Model
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DOI：
10.1097/brs.0b013e3181a54157
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发表时间：
2009-10-01
期刊：
影响因子：
3
通讯作者：
Meisel, Hans-Joerg
中科院分区：
文献类型：
--
作者：
Ganey, Timothy;Hutton, William C.;Meisel, Hans-Joerg
研究设计.使用细胞的椎间盘修复治疗。该研究的目的是检验使用自体脂肪组织来源的干细胞和再生细胞（ADRC）修复受损椎间盘是可能的这一假设。由急性或慢性重复性椎间盘损伤引起的退化导致椎间盘退变。然而，如果受损的椎间盘可以在早期阶段修复，在退化的冲击之前，那么椎间盘退变过程可以减缓。12只犬在3个腰椎节段（L3-L4、L4-L5和L5-L 6）进行部分髓核切除术;相邻节段作为非手术对照。使动物（或椎间盘）从手术中恢复6周。此时，收获皮下脂肪组织并分离ADRC。将经历部分髓核切除术的3个实验椎间盘随机接受：（1）透明质酸载体中的ADRC（细胞/HA）;（2）仅HA;或（3）无干预。使用MRI、放射照相术、组织学和生物化学对3个实验椎间盘和2个相邻的未接触椎间盘进行评估。分别于术后6个月和12个月处死动物。本研究中的修复通过组织学和生化分析特别证明。接受ADRCs的椎间盘水平更接近健康对照组，如基质透明性、纤维环区室化和髓核内细胞密度所证明。II型胶原蛋白和聚集蛋白聚糖的矩阵分析表明，与仅使用HA或无干预治疗相比，ADRCs对椎间盘的再生刺激在统计学上更好。如在该椎间盘损伤模型中使用的，自体脂肪组织来源的干细胞和再生细胞在促进椎间盘再生方面是有效的，如通过椎间盘基质产生和整体椎间盘形态所证明的。
Study Design. Therapeutic treatment of intervertebral disc repair using cells.Objective. The goal of the study was to test the hypothesis that repair of a damaged disc is possible using autologous adipose tissue derived stem and regenerative cells (ADRCs).Summary of Background Data. Degradation resulting from either acute or chronic repetitive disc injury leads to disc degeneration. However, if a damaged disc could be repaired in the early stages, before the onslaught of degradation, then the disc degeneration process may be slowed down.Methods. Twelve dogs underwent a partial nucleotomy at 3 lumbar levels (L3-L4, L4-L5, and L5-L6); adjacent levels served as nonoperated controls. The animals (or discs) were allowed to recover from the surgery for 6 weeks. At that time subcutaneous adipose tissue was harvested and ADRCs were isolated. The 3 experimental discs that had undergone a partial nucleotomy were randomized to receive: (1) ADRCs in hyaluronic acid carrier (Cells/HA); (2) HA only; or (3) No Intervention. Assessments of the 3 experimental discs plus the 2 adjacent untouched discs were made using MRI, radiography, histology, and biochemistry. The animals were killed at 6 months and at 12 months.Results. Repair in this study was specifically demonstrated through histology and biochemical analysis. Disc levels receiving ADRCs more closely resembled the healthy controls as evidenced in matrix translucency, compartmentalization of the anulus, and in cell density within the nucleus pulposus. Matrix analysis for Type-II collagen and aggrecan demonstrated evidence of a statistically better regenerative stimulation to the disc provided by ADRCs when compared to either the HA only or no intervention treatments.Conclusion. Autologous adipose tissue derived stem and regenerative cells, as used in this disc injury model, were effective in promoting disc regeneration, as evidenced by disc matrix production and overall disc morphology.