Screening and functional validation of lipid metabolism-related lncRNA-46546 based on the transcriptome analysis of early embryonic muscle tissue in chicken.
Screening and functional validation of lipid metabolism-related lncRNA-46546 based on the transcriptome analysis of early embryonic muscle tissue in chicken.
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基于鸡早期胚胎肌肉组织转录组分析的脂质代谢相关lncRNA-46546的筛选及功能验证
DOI：
10.5713/ab.21.0440
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发表时间：
2023-02
影响因子：
2.2
通讯作者：
Sun J
中科院分区：
文献类型：
--
作者：
Chen R;Liao K;Liao H;Zhang L;Zhao H;Sun J
目的利用高通量测序技术筛选鸡差异表达的长链非编码RNA（lncRNA），探讨其在肌内脂肪沉积中的作用机制。方法采用RNA测序技术，构建Rose crown和Cbb肉鸡胚胎胸肌和腿肌lncRNA和mRNA表达谱。在玫瑰冠和Cobb肉鸡的胸肌和腿肌中分别获得了96个和42个差异表达的lncRNA。lncRNA-ENSGALT 00000046546，具有高种间变异性和潜在的调节作用，在脂质代谢中，其预测的下游靶基因1-酰基甘油-3-磷酸-O-酰基转移酶2（AGPAT 2），被选择用于进一步研究的前脂肪细胞。结果lncRNA-46546在鸡前脂肪细胞2中的过表达显著增加（p<0.01）AGPAT 2及其下游基因二酰基甘油酰基转移酶1和二酰基甘油酰基转移酶2以及脂肪代谢相关基因过氧化物酶体增殖物激活受体γ、CCAAT/增强子结合蛋白α、脂肪酸合成酶、固醇调节元件结合转录因子1、和脂肪酸结合蛋白4。过表达组细胞内脂滴浓度高于对照组，细胞内和培养液中甘油三酯含量也显著增加（P<0.01）。结论本研究初步得出lncRNA-46546可能促进鸡肌内脂肪沉积的结论，为lncRNA在鸡早期胚胎发育和脂肪沉积中的研究奠定了基础。
Objective The study was conducted to screen differentially expressed long noncoding RNA (lncRNA) in chickens by high-throughput sequencing and explore its mechanism of action on intramuscular fat deposition. Methods Herein, Rose crown and Cbb broiler chicken embryo breast and leg muscle lncRNA and mRNA expression profiles were constructed by RNA sequencing. A total of 96 and 42 differentially expressed lncRNAs were obtained in Rose crown vs Cobb broiler chicken breast and leg muscle, respectively. lncRNA-ENSGALT00000046546, with high interspecific variability and a potential regulatory role in lipid metabolism, and its predicted downstream target gene 1-acylglycerol-3-phosphate-O-acyltransferase 2 (AGPAT2), were selected for further study on the preadipocytes. Results lncRNA-46546 overexpression in chicken preadipocyte 2 cells significantly increased (p<0.01) the expression levels of AGPAT2 and its downstream genes diacylglycerol acyltransferase 1 and diacylglycerol acyltransferase 2 and those of the fat metabolism-related genes peroxisome proliferator-activated receptor γ, CCAAT/enhancer binding protein α, fatty acid synthase, sterol regulatory element-binding transcription factor 1, and fatty acid binding protein 4. The lipid droplet concentration was higher in the overexpression group than in the control cells, and the triglyceride content in cells and medium was also significantly increased (p<0.01). Conclusion This study preliminarily concludes that lncRNA-46546 may promote intramuscular fat deposition in chickens, laying a foundation for the study of lncRNAs in chicken early embryonic development and fat deposition.
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影响因子：
46.9
作者：
Trapnell C;Williams BA;Pertea G;Mortazavi A;Kwan G;van Baren MJ;Salzberg SL;Wold BJ;Pachter L
通讯作者：
Pachter L
影响因子：
48
作者：
Langmead, Ben;Salzberg, Steven L.
通讯作者：
Salzberg, Steven L.
影响因子：
4.6
作者：
Sim, M. F. Michelle;Persiani, Elisa;Rochford, Justin J.
通讯作者：
Rochford, Justin J.
影响因子：
--
作者：
Ramanathan, N;Ahmed, M;Raffan, E;Stewart, C L;O'Rahilly, S;Semple, R K;Raef, H;Rochford, J J
通讯作者：
Rochford, J J
DOI：
10.1038/nrm.2017.104
发表时间：
2018-03
期刊：
Nature reviews. Molecular cell biology
影响因子：
--
作者：
Ransohoff JD;Wei Y;Khavari PA
通讯作者：
Khavari PA