Association of exogenous DNA with cattle and insect spermatozoa in vitro
Association of exogenous DNA with cattle and insect spermatozoa in vitro
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DOI：
10.1002/mrd.1080290102
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发表时间：
1991-05
影响因子：
2.5
通讯作者：
P. Atkinson;E. Hines;S. Beaton;K. Matthaei;K. C. Reed;M. Bradley
中科院分区：
文献类型：
--
作者：
P. Atkinson;E. Hines;S. Beaton;K. Matthaei;K. C. Reed;M. Bradley
从家牛（Bos taurus）、澳大利亚羊虻（Lucilia cuprina）和蜜蜂（Apis mellifera）中分离的精子能够结合外源放射性标记的线性DNA。运动和非运动牛精子都表现出四种不同的DNA关联模式。在用DNase I处理后，其中一种模式的相对比例在活精子中特别增加，这表明与牛精子相关的一小部分DNA可能被隔离在精子头部内。
Spermatozoa isolated from domestic cattle (Bos taurus), the Australian sheep blowfly (Lucilia cuprina) and the honeybee (Apis mellifera) are capable of binding exogenous radiolabeled linear DNA. Both motile and nonmotile bovine sperm exhibit four distinct patterns of DNA association. Following treatment with DNase I, the relative proportion of one of these patterns increases specifically in living sperm, suggesting that a small proportion of DNA that associates with bovine sperm may be sequestered within the sperm head.