EVALUATION OF HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHY FOR MEASUREMENT OF THE NEUTRAL SACCHARIDES IN NEUTRAL DETERGENT FIBER
EVALUATION OF HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHY FOR MEASUREMENT OF THE NEUTRAL SACCHARIDES IN NEUTRAL DETERGENT FIBER
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DOI：
10.1021/jf00117a001
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发表时间：
1983-01-01
影响因子：
6.1
通讯作者：
MARLETT, JA
中科院分区：
文献类型：
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作者：
SLAVIN, JL;MARLETT, JA
通过使用糖标准品和来自典型低纤维饮食和在摄入低纤维饮食期间收集的[人]粪便样品的NDF残留物的水解产物，评价了2个高效液相色谱柱测量中性洗涤剂纤维（NDF）水解产物中发现的6种中性单糖（葡萄糖、甘露糖、半乳糖、鼠李糖、阿拉伯糖和木糖以及纤维二糖）的能力。正相柱不能充分分离NDF中主要的己糖，而半乳糖和鼠李糖仅以少量存在，从重金属柱共洗脱。重金属柱的灵敏度比正相柱高2-3倍，可检出纤维二糖。重金属柱的检测能力和其他操作特性使其成为分离食物和粪便中常见的NDF的首选柱。
The ability of 2 high-performance liquid chromatographic columns to measure the 6 neutral monosaccharides, glucose, mannose, galactose, rhamnose, arabinose and xylose, and cellobiose found in neutral detergent fiber (NDF) hydrolysates was evaluated by using saccharide standards and hydrolysates of NDF residues from a typical low-fiber diet and from a [human] fecal sample collected during ingestion of the low-fiber diet. The normal-phase column did not adequately separate the hexoses, major saccharides in NDF, while galactose and rhamnose, present only in small quantities, coeluted from the heavy metal column. The heavy metal column was 2-3 times more sensitive than the normal-phase column and detected cellobiose. The detection capabilities and other operating properties of the heavy metal column make it the column of choice to separate saccharides commonly found in food and fecal NDF.