Roxithromycin downmodulates Th2 chemokine production by keratinocytes and chemokine receptor expression on Th2 cells: its dual inhibitory effects on the ligands and the receptors
Roxithromycin downmodulates Th2 chemokine production by keratinocytes and chemokine receptor expression on Th2 cells: its dual inhibitory effects on the ligands and the receptors
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DOI：
10.1016/j.cellimm.2004.03.011
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发表时间：
2004-03-01
影响因子：
4.3
通讯作者：
Tokura, Y
中科院分区：
文献类型：
--
作者：
Kobayashi, M;Shimauchi, T;Tokura, Y
罗红霉素(RXM)是一种抗菌大环内酯类药物，具有多种免疫调节活性。为了研究RXM对皮肤T淋巴细胞浸润的抑制作用，我们观察了RXM对角质形成细胞产生趋化因子和T细胞表达趋化因子受体的影响。用RXM和刺激剂培养正常人和HaCaT角质形成细胞。1或10um的RXM可显著抑制这些角质形成细胞Th2趋化因子MDC和TARC的产生/表达，但对IP-10的产生无明显影响。同时检测RXM对富含Th2的外周血淋巴细胞相应趋化因子受体T细胞表达的影响。RXM可降低IL-2促进的Th2趋化因子受体CCR4的表达，但对CXCR3的表达无明显影响。因此，RXM下调Th2的产生和受体的表达，但不下调参与皮肤免疫的Th1趋化因子，表明其对Th2介导的或过敏性皮肤病具有有益的治疗作用。(C)2004 Elsevier Inc.保留所有权利。
Roxithromycin (RXM), an anti-bacterial macrolide, has various immunomodulatory activities. To investigate the ability of RXM to downregulate skin-infiltration of T-lymphocytes, we examined the effects of RXM on keratinocyte production of chemokines and T cell expression of chemokine receptors. Normal human and HaCaT keratinocytes were cultured with RXM and stimulants. RXM at 1 or 10 muM significantly suppressed the production/expression of Th2 chemokines MDC and TARC in these keratinocytes, but the production of IP-10 was not affected. The effect of RXM on T-cell expression of the corresponding chemokine receptors was also tested in Th2-rich peripheral blood lymphocytes. The IL-2-enhanced expression level of Th2 chemokine receptor CCR4 was decreased by RXM at 10 muM, whereas the expression of CXCR3 was unchanged. Thus, RXM downmodulates both the production and receptor expression of Th2 but not Th1 chemokines involved in cutaneous immunity, Suggesting its beneficial therapeutic effects on Th2-mediated or allergic skin disorders. (C) 2004 Elsevier Inc. All rights reserved.