Molecular Characterization of a Debilitation-Associated Partitivirus Infecting the Pathogenic Fungus Aspergillus flavus
Molecular Characterization of a Debilitation-Associated Partitivirus Infecting the Pathogenic Fungus Aspergillus flavus
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感染病原真菌黄曲霉的衰弱相关病毒的分子特征
DOI：
10.3389/fmicb.2019.00626
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发表时间：
2019-03-28
影响因子：
5.2
通讯作者：
Yu, Wenfeng
中科院分区：
文献类型：
--
作者：
Jiang, Yinhui;Wang, Jingxian;Yu, Wenfeng
已知人类机会致病真菌黄曲霉可感染真菌病毒。在这项研究中，我们报告了一个新的真菌病毒A。黄病毒1型（AfPV 1）是从黄曲霉LD-3-8分离株中分离到的一种病毒。黄的AfPV 1具有直径约40 nm的球形病毒样颗粒，并且三个双链RNA（dsRNA）片段（dsRNA 1、2和3，长度分别为1.7、1.4和1.1 kbp）被包装在病毒粒子中。dsRNA 1、dsRNA 2和dsRNA 3均含有一个开放阅读框，分别编码62、42和32 kDa蛋白。dsRNA 1编码的蛋白质与部分病毒的RNA依赖性RNA聚合酶（RdRp）相似，而dsRNA 2编码的蛋白质与GenBank数据库中的其他衣壳蛋白（CP）没有显著的相似性，只是与一些部分病毒的“衣壳”蛋白有一定的同源性。dsRNA 3编码的蛋白质与GenBank数据库中的任何蛋白质都没有相似性。SDS-PAGE和多肽质量指纹-质谱（PMF-MS）分析表明，AfPV 1的CP由dsRNA 2编码。系统发育分析表明，AfPV 1及其相关病毒属于偏病毒科（Partitiviridae）的一个未分类群。AfPV 1似乎导致虚弱症状，但对小鼠致病性没有显著影响。这些发现为分离病毒的分类以及病毒与A.黄的
The opportunistic human pathogenic fungus Aspergillus flavus is known to be infected with mycoviruses. In this study, we report a novel mycovirus A. flavus partitivirus 1 (AfPV1) that was originally isolated from the abnormal colonial morphology isolate LD-3-8 of A. flavus. AfPV1 has spherical virus-like particles about 40 nm in diameter, and three double-stranded RNA (dsRNA) segments (dsRNA1, 2, and 3 with lengths of 1.7, 1.4, and 1.1 kbp, respectively) were packaged in the virions. dsRNA1, dsRNA2, and dsRNA3 each contained a single open reading frame and potentially encoded 62, 42, and 32 kDa proteins, respectively. The dsRNA1 encoded protein shows similarity to the RNA-dependent RNA polymerase (RdRp) of partitiviruses, and the dsRNA2 product has no significant similarity to any other capsid protein (CP) in the GenBank databases, beside some homology with the hypothetical “capsid” protein of a few partitiviruses. The dsRNA3 encodes a protein with no similarity to any protein in the GenBank database. SDS-PAGE and polypeptide mass fingerprint-mass spectrum (PMF-MS) analyses indicated that the CP of the AfPV1 was encoded by dsRNA2. Phylogenetic analysis showed that the AfPV1 and relative viruses were found in an unclassified group inside the Partitiviridae family. AfPV1 seems to result in debilitation symptoms, but had no significant effects to murine pathogenicity. These findings provide new insights into the partitiviruses taxonomy and the interactions between viruses and A. flavus.