Catalytic heterogeneity of polyclonal DNA-hydrolyzing antibodies from the sera of patients with multiple sclerosis
Catalytic heterogeneity of polyclonal DNA-hydrolyzing antibodies from the sera of patients with multiple sclerosis
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DOI：
10.1016/s0165-2478(01)00185-7
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发表时间：
2001-04-02
影响因子：
4.4
通讯作者：
Nevinsky, GA
中科院分区：
文献类型：
--
作者：
Baranovskii, AG;Ershova, NA;Nevinsky, GA
最近在几种自身免疫性疾病患者的血清中检测到了各种催化抗体或抗体酶，它们的存在很可能与自身免疫有关。最近，我们已经表明，DNA酶活性与IgG从多发性硬化症（MS）患者的血清，但不与正常人的血清。在这里，我们提出的证据表明，MS IgC，其F（ab）片段，和分离的L-链催化DNA水解。这些多克隆IgG的DNA酶活性的性质将它们与其他已知的人DNA酶区分开来。此外，它们与不同寡核苷酸底物的比活性以及最佳pH范围、表观K-M值和底物特异性对于不同患者有很大差异。这些发现支持个体患者的免疫系统产生各种多克隆催化IgG池，从相对较小到非常大。(C)2001爱思唯尔科技有限公司。保留所有权利。
Various catalytic antibodies or abzymes have been detected recently in the sera of patients with several autoimmune pathologies, where their presence is most probably associated with autoimmunization. Recently we have shown that DNase activity is associated with IgGs from the sera of patients with multiple sclerosis (MS) but not with those from the sera of normal humans. Here we present evidence showing that MS IgC, its F(ab) fragments, and separated L-chains catalyze DNA hydrolysis. The properties of the DNase activity of these polyclonal IgGs distinguish them from other known human DNases. In addition, their specific activities with different oligonucleotide substrates and the range of optimal pHs, apparent K-M values and substrate specificities varied widely for different patients. The findings speak in favor of the generation by the immune systems of individual patients of a variety of polyclonal catalytic IgG pools, from relatively small to extremely large ones. (C) 2001 Elsevier Science B.V. All rights reserved.