The Genes Encoding Major Surface Proteins of Borrelia burgdorferi Are Located on a Plasmid a
The Genes Encoding Major Surface Proteins of Borrelia burgdorferi Are Located on a Plasmid a
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编码伯氏疏螺旋体主要表面蛋白的基因位于质粒a上
DOI：
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发表时间：
1988
影响因子：
5.2
通讯作者：
C. Garon
中科院分区：
文献类型：
--
作者：
A. Barbour;C. Garon
虽然导致莱姆疏螺旋体病的螺旋体是在近十年才被发现的，但疏螺旋体属却是最近十年才发现的。一个多世纪前就发现了导致流行性和地方性回归热的病原体（参考文献 1 进行了综述）。尽管微生物学家长期以来对这些微生物非常熟悉，但人们对这一真细菌属的遗传学却知之甚少。与对革兰氏阴性和革兰氏阳性细菌的了解相比，对疏螺旋体遗传机制的了解还很贫乏。信息匮乏的一个重要原因是在体外培养这组病原生物体的困难。它们在肉汤培养基中生长缓慢并且不愿意在固体培养基上形成菌落？随着莱姆疏螺旋体病作为一种人类和家畜疾病在北美、欧洲和亚洲的影响越来越大，我们有充分的理由破译疏螺旋体遗传学的独特特征。我们对螺旋体遗传学的兴趣源于对回归热伯氏疏螺旋体属赫氏疏螺旋体抗原变异的一系列研究。我们发现，感染小鼠的疏螺旋体群体中血清型的变化可归因于螺旋体的主要外壳蛋白被另一种抗原上不同的外壳蛋白取代。这种变异似乎是在转录水平上决定的。~~”因此，我们询问基因组中是否存在任何可检测到的变化。原来是有的。在基因组中发现了编码外壳蛋白的基因的表达连锁拷贝和沉默拷贝。血清型的变化与基因的沉默拷贝转座到表达位点有关。6在这些研究过程中，我们发现血清型特异性表面蛋白的基因位于赫氏芽孢杆菌的染色体外元件上。
Whereas the spirochetes causing Lyme borreliosis were discoverd only this decade, the Borrelia spp. that causes epidemic and endemic relapsing fever were discovered more than a century ago (reviewed in ref. 1). Despite this long familiarity of microbiologists with these microorganisms, very little is known about the genetics of this genus of eubacteria. In comparison to what is understood about gram-negative and gram-positive bacteria, knowledge of the hereditary mechanisms of borreliae is meager. An important reason for this paucity of information has been the difficulty in growing this group of pathogenic organisms in vitro. They grow slowly in broth medium and are reluctant to form colonies on solid media? With the increasing impact of Lyme borreliosis as a human and domestic animal disease in North America, Europe, and Asia, there are compelling reasons to decipher the unique features of borrelial genetics. Our own interest in spirochetal genetics dates from a series of investigations into antigenic variation of the relapsing fever Borrelia species hermsii. We had found that the change in serotype in a population of borreliae infecting a mouse was attributable to the replacement of the predominant coat protein of the spirochetes by another, antigenically distinct, coat protein.’ This variation appeared to be determined at the level of trans~ription.~~’ Consequently, we asked whether there were any detectable changes in the genome. It turned out that there were. Expression-linked and silent copies of the genes encoding the coat proteins were to be found in the genome. Change in serotype was associated with the transposition of a silent copy of a gene to an expression site.6 In the course of these studies, we found that the genes for the serotype-specific surface proteins were located on extrachromosomal elements in B. hermsii.’
腺病毒 2 基因组的核苷酸序列。
DOI：
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发表时间：
1982
期刊：
The Journal of biological chemistry
影响因子：
--
作者：
Gingeras,TR;Sciaky,D;Gelinas,RE;Bing-Dong,J;Yen,CE;Kelly,MM;Bullock,PA;Parsons,BL;O'Neill,KE;Roberts,RJ
通讯作者：
Roberts,RJ