Hemorheological alterations of red blood cells induced by non-thermal dielectric barrier discharge plasma
Hemorheological alterations of red blood cells induced by non-thermal dielectric barrier discharge plasma
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DOI：
10.1063/1.4967451
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发表时间：
2016-11
影响因子：
4
通讯作者：
Jeongho Kim;Jae Hyung Kim;B. Chang;E. Choi;Hun-Kuk Park
中科院分区：
文献类型：
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作者：
Jeongho Kim;Jae Hyung Kim;B. Chang;E. Choi;Hun-Kuk Park
常压低温等离子体在伤口愈合、感染组织杀菌、血液凝固、微创手术等方面有着广泛的应用。低温等离子体产生包括臭氧在内的活性氧物种(ROS)。不同的研究小组已经报道，产生的ROS影响细胞的增殖和分化，以及肿瘤细胞的凋亡和生长停滞。在本研究中，我们研究了低温等离子体对红细胞(RBC)流变性的影响。我们通过实验测量了红细胞(RBC)的溶血、变形性和聚集性与非热等离子体暴露时间的关系。用场发射扫描电子显微镜观察了红细胞形态。红细胞释放出的血红蛋白的吸光度随着低温等离子体暴露时间的增加而增加。伸长指数和聚集指数随温度的增加而显著降低。
Atmospheric pressure non-thermal plasma has been introduced in various applications such as wound healing, sterilization of infected tissues, blood coagulation, delicate surgeries, and so on. The non-thermal plasma generates reactive oxygen species (ROS), including ozone. Various groups have reported that the produced ROS influence proliferation and differentiation of cells, as well as apoptosis and growth arrest of tumor cells. In this study, we investigated the effects of non-thermal plasma on rheological characteristics of red blood cells (RBC). We experimentally measured the extent of hemolysis, deformability, and aggregation of red blood cells (RBC) with respect to exposure times of non-thermal plasma. RBC morphology was also examined using field-emission scanning electron microscopy. The absorbance of hemoglobin released from the RBCs increased with increasing exposure time of the non-thermal plasma. Values of the elongation index and aggregation index were shown to decrease significantly with incre...