Lateral packing of protofibrils in fibrin fibers and fibrinogen polymers.
Lateral packing of protofibrils in fibrin fibers and fibrinogen polymers.
复制标题
纤维蛋白纤维和纤维蛋白原聚合物中原纤维的横向堆积。
DOI：
10.1002/bip.360251213
复制
发表时间：
1986
期刊：
影响因子：
2.9
通讯作者：
Erickson,HP
中科院分区：
文献类型：
--
作者：
Voter,WA;Lucaveche,C;Blaurock,AE;Erickson,HP
纤维蛋白纤维独特的横向带状图案清楚地表明分子在纵向上的排序。在这项研究中，我们通过薄片电子显微镜检查了纤维蛋白凝块的纤维以及两种类型的纤维蛋白原聚合物。纤维蛋白原聚合物具有与纤维蛋白纤维相同的横向带状图案，清楚地表明有规则的纵向重复，但它们的直径较大，并且很少或没有支化。因此，我们预计它们的整体排序比纤维蛋白纤维更好。使用了几种不同的固定方案。我们很容易观察到以前通过负染色和金属复制技术看到的典型横向带。然而，在任何样品中都很少看到任何规则的横向晶格。使用 X 射线衍射来检查两种纤维蛋白原聚合物的未固定样本，尽管纵向重复很明显，但很少看到横向结晶的证据。电子显微镜和 X 射线结果表明，纤维蛋白原的针状纤维和颗粒纤维具有与粗纤维蛋白纤维基本相同的内部结构，并且所有三种类型的聚合物虽然具有明显的横向带状，但其侧向原纤维堆积几乎没有结晶度。
The distinctive transverse banding pattern of fibrin fibers clearly indicates ordering of molecules in the longitudinal direction. In this study we examined the fibers of fibrin clots, as well as two types of fibrinogen polymers, by thin‐section electron microscopy. The fibrinogen polymers have a transverse banding pattern identical to that of fibrin fibers—clearly indicating a regular longitudinal repeat—but they are larger in diameter, and show little or no branching. We therefore expected their overall ordering to be better than that of fibrin fibers. Several different fixation protocols were used. We readily observed the typical transverse banding seen previously by negative stain and metal replication techniques. However, only very rarely was any regular lateral lattice seen in any of the samples. X‐ray diffraction was used to examine unfixed specimens of the two fibrinogen polymers and, once again, although a longitudinal repeat was evident, only rarely was evidence for lateral crystallinity seen. The electron‐microscope and x‐ray results showed that the needles and pellet fibers of fibrinogen have essentially the same internal architecture as thick fibrin fibers, and that all three types of polymer, although clearly transversely banded, have almost no crystallinity in their lateral protofibril packing.