Characterization of tomato zonate spot virus, a new tospovirus in China
Characterization of tomato zonate spot virus, a new tospovirus in China
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DOI：
10.1007/s00705-008-0054-5
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发表时间：
2008-05-01
影响因子：
2.7
通讯作者：
Zhang, Zhong-Kai
中科院分区：
文献类型：
--
作者：
Dong, Jia-Hong;Cheng, Xiao-Fei;Zhang, Zhong-Kai
对一株新分离株的形态、寄主范围和血清学特性进行了研究，该分离物在果实上引起同心环斑，在受感染植物的叶片上引起坏死病。该病毒的大(L)、中(M)和小(S)RNA的全序列分别为8919、4945和3279个核苷酸。L核糖核酸编码依赖核糖核酸的核糖核酸聚合酶(RdRp)，全长2885aa，332.7 kDa。MRNA编码一个非结构蛋白(Nsm)(309aa，34.4 kDa)和一个病毒糖蛋白前体(Gn/GC)(1122aa，127.4 kDa)。S核糖核酸编码459aa、51.9kDa的非结构蛋白和278aa、30.6kDa的N蛋白。该N蛋白与马蹄莲褪绿斑病毒的氨基酸同源性为80.9%。我们的结果表明，该病毒属于一个新的TospoVirus种，建议将其命名为番茄带斑病毒。
An isolate of a new tospovirus species, causing concentric zoned ringspots on fruits and necrotic lesions on leaves of infected plants, was characterised based on particle morphology, host range and serological properties. The complete nucleotide sequences of large (L), medium (M), and small (S) RNAs of this virus were found to contain 8919, 4945, and 3279 nts respectively. The L RNA encoded the RNA-dependent RNA polymerase (RdRp) (2885 aa, 332.7 kDa). The M RNA encoded a non-structural (NSm) protein (309 aa, 34.4 kDa) and a viral glycoprotein precursor (Gn/Gc) (1122 aa, 127.4 kDa). The S RNA encoded a non-structural protein (NSs) (459 aa, 51.9 kDa) and the nucleocapsid (N) protein (278 aa, 30.6 kDa). This N protein shared amino acid identities of 80.9% with those of calla lily chlorotic spot virus. Our results suggest that the virus studied here belongs to a new tospovirus species, for which the name tomato zonate spot virus is proposed.