Discrimination of T, B, and null lymphocytes by electronic sizing.
Discrimination of T, B, and null lymphocytes by electronic sizing.
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通过电子尺寸区分 T、B 和无效淋巴细胞。
DOI：
10.1111/j.1399-0039.1980.tb00195.x
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发表时间：
1980
期刊：
影响因子：
--
通讯作者：
Mickey,MR
中科院分区：
文献类型：
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作者：
El-Awar,N;Terasaki,PI;Perdue,S;Cicciarelli,J;Mickey,MR
根据Coulter计数器大小分析，通过绵羊红细胞（SRBC）花环或粘附于尼龙羊毛柱分离的T、B和空淋巴细胞具有明显的大小差异。以微米计的直径如下：B = 6.42，T = 6.76，null = 7.16，粒细胞= 8.42，单核细胞= 9.02。将细胞制备物的Coulter粒度分布与这些制备物中具有E、EAC和表面免疫球蛋白（SIG）标记的细胞比例以及它们对HLA‐DR抗血清和兔抗B‐淋巴细胞抗血清的反应性进行比较。通过Coulter粒度分析监测的细胞制备物在混合淋巴细胞培养（MLC）试验中产生了预期结果。也就是说，T细胞有应答但不刺激，而B淋巴细胞和单核细胞有刺激但不应答。空巨噬细胞既刺激又响应。在抗体依赖性细胞介导的细胞毒性（ADCC）中，无效淋巴细胞和单核细胞作为效应子发挥作用，而T淋巴细胞和粒细胞不起作用。B淋巴细胞制剂的活性居中。库尔特粒度分析是一种快速、简单的方法，可用于评估富含T、B和空淋巴细胞的悬浮液的纯度。
T, B, and null lymphocytes isolated by sheep red blood cells (SRBC) rosetting or adherence to a nylon wool column had distinct size differences according to Coulter counter size analysis. The diameters in microns were as follows: B = 6.42, T = 6.76, null = 7.16, granulocytes = 8.42, and monocytes = 9.02. Coulter size distributions of cell preparations were compared to the proportions of cells with E, EAC, and surface immunoglobulin (SIg) markers in these preparations as well as their reactivity to HLA‐DR antisera and rabbit anti‐B‐lymphocyte antisera.Cell preparations monitored by Coulter sizing produced the expected results in mixed lymphocyte culture (MLC) testing. That is, T cells responded but did not stimulate whereas B lymphocytes and monocytes stimulated but did not respond. NULL lympocytes both stimulated and responded. In antibody‐dependent cell‐mediated cytotoxicity (ADCC), null lymphocytes and monocytes functioned as effectors, whereas Tlymphocytes and granulocytes did not.B lymphocyte preparations were intermediate in activity. Coulter sizing is a rapid, simple means of assessing the purity of suspensions enriched for T, B, and null lymphocytes.