A homologue of platelet-derived growth factor produced by rat alveolar macrophages.
A homologue of platelet-derived growth factor produced by rat alveolar macrophages.
复制标题
大鼠肺泡巨噬细胞产生的血小板衍生生长因子的同系物。
DOI：
10.1096/fasebj.2.7.3280379
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发表时间：
1988
期刊：
影响因子：
--
通讯作者：
Brody,AR
中科院分区：
文献类型：
--
作者：
Kumar,RK;Bennett,RA;Brody,AR
大鼠肺泡巨噬细胞分泌一种生长因子，使大鼠肺成纤维细胞能够在缺乏血小板的血浆存在下启动体外DNA合成。这种生物活性类似于血小板衍生生长因子（PDGF）。在酸性条件下通过色谱法与推定的相关结合蛋白分离后，巨噬细胞衍生因子显示出与高度纯化的人PDGF相似的相对分子量。该因子与人PDGF的单特异性抗体结合，因此可以在PDGF的酶免疫测定中定量。它与放射性标记的人PDGF竞争大鼠肺成纤维细胞上PDGF的受体位点，并且与这些受体位点的结合可被抗PDGF特异性抑制。这些数据有力地支持了大鼠肺泡巨噬细胞衍生的因子与人PDGF同源并且与人巨噬细胞衍生的PDGF样生长因子相似的观点。此外，我们已经证明，肺含有这种细胞因子的效应细胞（肺巨噬细胞）和潜在的靶细胞（间质成纤维细胞）。因此，大鼠似乎是研究巨噬细胞衍生的PDGF样生长因子作为肺纤维化增殖介质的合适动物模型。库马尔河，巴西-地K.的;班尼特河一、布罗迪，A. R. A homologue of platelet‐derived growth factor produced by rat alveolar macrophages.FASEB J.2：2272 - 2277; 1988.
Rat alveolar macrophages secrete a growth factor that renders rat lung fibroblasts competent to initiate DNA synthesis in vitro in the presence of platelet‐poor plasma. This biological activity resembles that of platelet‐derived growth factor (PDGF). After separation from putative associated binding proteins by chromatography under acidic conditions, the macrophage‐derived factor exhibited a relative molecular weight similar to that of highly purified human PDGF. The factor bound to a monospecific antibody to human PDGF and thus could be quantitated in an enzyme immunoassay for PDGF. It competed with radiolabeled human PDGF for receptor sites for PDGF on rat lung fibroblasts, and binding to these receptor sites could be specifically inhibited by anti‐PDGF. These data strongly support the view that the factor derived from rat alveolar macrophages is homologous to human PDGF and is similar to human macrophage‐derived PDGF‐like growth factor. Furthermore, we have demonstrated that the lung contains both an effector cell (pulmonary macrophage) and a potential target cell (interstitial fibroblast) for this cytokine. Therefore the rat appears to be an appropriate animal model in which to study macrophage‐derived PDGF‐like growth factors as mediators of proliferation in pulmonary fibrogenesis.— Kumar, R. K.; Bennett, R. A.; Brody, A. R. A homologue of platelet‐derived growth factor produced by rat alveolar macrophages.FASEB J.2: 2272‐2277; 1988.