SEQUENCE-SPECIFIC CLEAVAGE OF DOUBLE HELICAL DNA BY TRIPLE HELIX FORMATION
SEQUENCE-SPECIFIC CLEAVAGE OF DOUBLE HELICAL DNA BY TRIPLE HELIX FORMATION
复制标题
DOI：
10.1126/science.3118463
复制
发表时间：
1987-10-30
期刊：
影响因子：
56.9
通讯作者：
DERVAN, PB
中科院分区：
文献类型：
--
作者：
MOSER, HE;DERVAN, PB
在单一位置连接有EDTA双Fe的同型嘧啶寡脱氧核糖核苷酸通过三螺旋形成结合大的双链DNA内的相应同型嘧啶-同型嘌呤段，并在该位点切割。在5′端具有EDTA螯合Fe的寡核苷酸引起序列特异性双链断裂。切割模式的位置和不对称性揭示了高嘧啶-EDTA探针结合在Watson-Crick双螺旋DNA的与高嘌呤链平行的大沟中。同嘧啶探针对双螺旋DNA的序列特异性识别对单碱基错配敏感。高嘧啶探针与DNA切割部分配备可能是有用的工具，染色体定位。
Homopyrimidine oligodeoxyribonucleotides with EDTA⋅Fe attached at a single position bind the corresponding homopyrimidine-homopurine tracts within large double-stranded DNA by triple helix formation and cleave at that site. Oligonucleotides with EDTA⋅Fe at the 5′ end cause a sequence specific double strand break. The location and asymmetry of the cleavage pattern reveal that the homopyrimidine-EDTA probes bind in the major groove parallel to the homopurine strand of Watson-Crick double helical DNA. The sequence-specific recognition of double helical DNA by homopyrimidine probes is sensitive to single base mismatches. Homopyrimidine probes equipped with DNA cleaving moieties could be useful tools for mapping chromosomes.