Effect of an experimental oil spill on vertebral bone tissue quality in European sea bass (Dicentrarchus labrax L.)
Effect of an experimental oil spill on vertebral bone tissue quality in European sea bass (Dicentrarchus labrax L.)
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DOI：
10.1016/j.ecoenv.2011.07.027
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发表时间：
2011-10-01
影响因子：
6.8
通讯作者：
Sire, Jean-Yves
中科院分区：
文献类型：
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作者：
Danion, Morgane;Deschamps, Marie-Helene;Sire, Jean-Yves
为了确定鱼类中石油污染的生物标志物，我们测试了实验性的光循环油（LCD）暴露对海鲈鱼（Dicentrarchus labrax L.）椎骨的影响。共60条成鱼驯化15天，然后收集20条作为对照（第0天），而40条暴露于LCO（1136 ng L-1的10种多环芳烃，多环芳烃）的可溶性组分7天。其中20个样本是在接触期结束时取样的，最后20个样本是在清洁海水中经过14天的恢复期后取样的。脊椎异常进行了计数和骨矿化，总骨面积和骨密度的配置文件，建立了几个后颅和尾vertebrals.In海鲈鱼，7天的LCO暴露没有影响的频率和严重程度的脊椎异常。在未接触（第0天）、接触（第7天）和净化（第21天）的鱼之间，未观察到骨密度和骨再分配（骨面积分布参数）的显著差异。相比之下，受污染的海鲈鱼的椎骨的骨矿化减少，但以可逆的方式，这证实了先前在鳟鱼中的研究表明，该参数是早期应力指标。我们的研究结果表明，脊椎骨矿化可以作为一个生物标志物的多环芳烃污染的鲈鱼。在暴露于各种异生物质的其他硬骨鱼物种中检查这种新的生物标志物将是有趣的。皇冠版权所有（C）2011由爱思唯尔公司出版。All rights reserved.
In order to identify biomarkers of oil pollution in fish we tested the effects of an experimental Light Cycle Oil (LCD) exposure on vertebral bone of sea bass, Dicentrarchus labrax L. A total of 60 adult fish were acclimated for fifteen days, then twenty were collected as controls (Day 0) while 40 were exposed to a soluble fraction of LCO (1136 ng L-1 of ten Polycyclic Aromatic Hydrocarbons, PAHs) for seven days. Twenty of them were sampled at the end of the exposure period and the twenty last after a recovery period of fourteen days in clean seawater. Vertebral abnormalities were counted and bone mineralization, total bone area and bone density profiles were established for several post-cranial and caudal vertebrae.In sea bass, seven days of LCO exposure did not affect the frequency and severity of the vertebral abnormalities. No significant differences were observed in bone density and bone repartition (parameters of bone area profiles) between unexposed (Day 0), exposed (D7) and decontaminated (D21) fish. In contrast, bone mineralization of the vertebrae decreased in contaminated sea bass, but in a reversible way, which confirms a previous study in trout showing that this parameter is an early stress indicator. Our results suggest that vertebral bone mineralization could be used as a biomarker of PAH pollution in sea bass. It would be interesting to check this new biomarker in other teleost species exposed to various xenobiotics. Crown Copyright (C) 2011 Published by Elsevier Inc. All rights reserved.