AN IMMOBILIZED ENZYME MEMBRANE FABRICATION METHOD USING AN INK JET NOZZLE
AN IMMOBILIZED ENZYME MEMBRANE FABRICATION METHOD USING AN INK JET NOZZLE
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DOI：
10.1016/0265-928x(89)80033-1
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发表时间：
1989-01-01
期刊：
影响因子：
--
通讯作者：
KURIYAMA, T
中科院分区：
文献类型：
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作者：
KIMURA, J;KAWANA, Y;KURIYAMA, T
提出了一种新的酶固定化方法，实现了酶在生物传感器中的高效利用。最初为打印设备开发的喷墨喷嘴被用作将酶精确沉积到ISFET器件上的工具。尝试了两种固定化方法：一种是液相固定化，其中酶溶液首先发射到ISFET传感器区域上。室温干燥后，将戊二醛溶液喷入酶膜中进行固定化。在该方法中，膜在中心周围的区域中变得非常薄（例如小于0.1 μ m）。另外，由于酶溶液再溶解到戊二醛溶液中，并且发生在周边区域的膜定位，因此膜厚度控制相当困难。接下来，对该方法进行了改进，以保持原始酶滴的形状：气相固定化。首先，将酶溶液滴发射到ISFET装置上，使用由个人计算机控制的X-Y台精确确定ISFET装置的位置。酶的固定化是通过将该装置保持在充满戊二醛蒸汽的室中来实现的。在这种情况下，所得的膜形状保持了原始的酶形状。这些方法在非常昂贵的酶固定化和在传感器芯片上具有各种固定化酶膜的多生物传感器的制造中具有很大的优点。
A novel enzyme immobilization method, which realized the efficient use of enzymes for biosensors, was developed. An ink jet nozzle, originally developed for printing equipment, was used as a tool for precise enzyme deposition onto an ISFET device. Two sorts of immobilization method were attempted: one is liquid phase immobilization, in which an enzyme solution was emitted at first onto an ISFET sensor region. After drying it under room temperature, glutaraldehyde solution was emitted into the enzyme membrane for immobilization. In this method, the membrane became very thin (e.g. less than 0.1 .mu.m) in a region around the center. In addition, membrane thickness control was rather difficult because the enzyme solution redissolved into the glutaraldehyde solution, and membrane localization at the peripheral region occurred. Next, the method was improved so as to maintain the shape of the original enzyme drops: gas phase immobilization. At first, the enzyme solution drops were emitted onto an ISFET device whose position was precisely determined using an X-Y stage controlled by a personal computer. Enzyme immobilization was realized by keeping the device in a chamber filled with glutaraldehyde vapor. In this case, the resulting membrane shape maintains the original enzyme shape. These methods will have great merit in very expensive enzyme immobilization and in the fabrication of a multi-biosensor, which has various kinds of immobilized enzyme membranes on a sensor chip.