THE ROLE OF MACROPHAGES IN SKELETAL-MUSCLE REGENERATION WITH PARTICULAR REFERENCE TO CHEMOTAXIS
THE ROLE OF MACROPHAGES IN SKELETAL-MUSCLE REGENERATION WITH PARTICULAR REFERENCE TO CHEMOTAXIS
复制标题
DOI：
10.1006/excr.1993.1199
复制
发表时间：
1993-08-01
影响因子：
3.7
通讯作者：
PAPADIMITRIOU, JM
中科院分区：
文献类型：
--
作者：
ROBERTSON, TA;MALEY, MAL;PAPADIMITRIOU, JM
这项研究的结果表明，来自受损肌纤维的趋化因子(s)吸引多形核白细胞（PML）和巨噬细胞到损伤部位，而渗出的巨噬细胞而不是PML在肌原细胞中诱导强烈的阳性趋化反应。血小板衍生生长因子（PDGF）、转化生长因子-β (TGF-β)、碱性成纤维细胞生长因子（bFGF）和白血病抑制因子（LIF）（所有这些都是由巨噬细胞分泌的）的AB和BB亚型也被证明是肌肉前体细胞（MPC）的化学引诱剂。PDGF的AA异构体似乎对MPC没有任何趋化作用。在组织培养条件下，巨噬细胞也显示出刺激MPC增殖的作用。PDGF、bFGF和TGF-β的BB异构体也有类似的有丝分裂作用，但PDGF的AA或AB异构体和LIF则没有。结果表明，巨噬细胞在肌纤维重建的协调中起着关键作用。
The results from this investigation suggest that chemotactic factor(s) from damaged myofibers attract polymorphonuclear leukocytes (PML) and macrophages to the site of injury, while exudate macrophages but not PML induce a strong positive chemotactic response in myogenic cells. The AB and BB isoforms of platelet-derived growth factor (PDGF), transforming growth factor-β (TGF-β), basic fibroblast growth factor (bFGF), and leukemia inhibitory factor (LIF) (all of these are secreted by macrophages) were also shown to be chemoattractants for muscle precursor cells (MPC). The AA isoform of PDGF did not appear to have any such chemotactic effect on MPC. Macrophages were also shown, under tissue culture conditions, to stimulate the proliferation of MPC. This mitogenic effect was similarly demonstrated for the BB isoform of PDGF, bFGF, and TGF-β but not for the AA or AB isoforms of PDGF nor for LIF. The results indicate that macrophages play a pivotal role in the orchestration of muscle fiber reconstitution.