SPR sensing of small molecules with modified RNA aptamers: Detection of neomycin B
SPR sensing of small molecules with modified RNA aptamers: Detection of neomycin B
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DOI：
10.1016/j.bios.2009.01.011
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发表时间：
2009-04-15
影响因子：
12.6
通讯作者：
Tunon-Blanco, Paulino
中科院分区：
文献类型：
--
作者：
de-los-Santos-Alvarez, Noemi;Lobo-Castanon, Maria Jesus;Tunon-Blanco, Paulino
我们研究了在核糖 2' 位置上带有甲基的 RNA 适体针对新霉素 B 的修饰如何影响相互作用的亲和力。使用表面等离子体共振（SPR）和法拉克拉阻抗谱（FIS）计算μM范围内的亲和常数。结果表明，适配体的修饰并没有显着改变适配体与抗生素的亲和力。这一发现为设计修饰的RNA适体提供了可能性，该适体对核酸内切酶具有抗性，且分析特征不发生变化。除此之外，我们还提出了一种使用 SPR 作为转导技术来检测新霉素 B 的竞争性测定方法。获得了10 nM至100 μM之间的定量范围，这表明使用适配体高灵敏度检测小分子的可行性。 (c) 2009 Elsevier B.V. 保留所有权利。
We have studied how the modification of the RNA aptamer evolved against neomycin B at 2' position of ribose with a methyl group influences the affinity of the interaction. Using surface plasmon resonance (SPR) and faraclaic impedance spectroscopy (FIS) an affinity constant in the mu M range was calculated. The results showed that the modification of the aptamer does not significantly alter the affinity of the aptamer for the antibiotic. This finding opens up the possibility of designing modified RNA aptamers resistant to endonucleases without variation of the analytical features. In addition to this, we propose a competitive assay for the detection of neomycin B using SPR as a transduction technique. A range of quantification between 10 nM and 100 mu M was obtained, which shows the feasibility of detecting small molecules using aptamers with high sensitivity. (c) 2009 Elsevier B.V. All rights reserved.