Soluble ST2 suppresses the effect of interleukin-33 on lung type 2 innate lymphoid cells.
Soluble ST2 suppresses the effect of interleukin-33 on lung type 2 innate lymphoid cells.
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DOI：
10.1016/j.bbrep.2016.02.002
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发表时间：
2016-03
影响因子：
2.7
通讯作者：
Tominaga SI
中科院分区：
文献类型：
--
作者：
Hayakawa H;Hayakawa M;Tominaga SI
肺中的2型先天性淋巴样细胞（ILC 2）响应于IL-33而产生白细胞介素（IL）-5和IL-13，并且可能有助于过敏性疾病如哮喘的发展。然而，对控制ILC 2功能的负调控因子和有效抑制剂知之甚少。在这里，我们表明，可溶性ST 2，IL-1受体家族的成员，抑制IL-33对肺ILC 2在体外的影响。用IL-33刺激幼稚ILC 2诱导形态学改变并促进细胞增殖。此外，IL-33上调包括IL-33受体在内的细胞表面分子的表达，并诱导IL-5和IL-13的产生，但不诱导IL-4的产生。用可溶性ST 2预处理抑制IL-33介导的ILC 2应答。结果表明，可溶性ST 2充当IL-33的诱饵受体，并保护ILC 2免受IL-33刺激。IL-33刺激上调肺ILC 2中IL-33受体的表达。可溶性ST 2作为IL-33的诱饵受体发挥作用。可溶性ST 2保护幼稚肺ILC 2免受IL-33的刺激。
Type 2 innate lymphoid cells (ILC2) in lungs produce interleukin (IL)-5 and IL-13 in response to IL-33 and may contribute to the development of allergic diseases such as asthma. However, little is known about negative regulators and effective inhibitors controlling ILC2 function. Here, we show that soluble ST2, a member of the IL-1 receptor family, suppresses the effect of IL-33 on lung ILC2 in vitro. Stimulation with IL-33 to naïve ILC2 induced morphological change and promoted cell proliferation. In addition, IL-33 upregulated expression of cell surface molecules including IL-33 receptor and induced production of IL-5 and IL-13, but not IL-4. Pretreatment with soluble ST2 suppressed IL-33-mediated responses of ILC2. The results suggest that soluble ST2 acts as a decoy receptor for IL-33 and protects ILC2 from IL-33 stimulation. Expression of IL-33 receptor in lung ILC2 is upregulated by IL-33 stimulation. Soluble ST2 functions as a decoy receptor for IL-33. Soluble ST2 protects naïve lung ILC2 from stimulation by IL-33.