ZP3-dependent activation of sperm cation channels regulates acrosomal secretion during mammalian fertilization.
ZP3-dependent activation of sperm cation channels regulates acrosomal secretion during mammalian fertilization.
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DOI：
10.1083/jcb.134.3.637
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发表时间：
1996-08
期刊：
影响因子：
--
通讯作者：
Florman HM
中科院分区：
文献类型：
--
作者：
Arnoult C;Zeng Y;Florman HM
精子顶体反应是受精所必需的Ca（2+）依赖性分泌事件。粘附到卵的透明质膜上，通过电压敏感通道促进Ca2+内流，从而启动分泌。我们使用电位荧光探针来确定精子膜电位在调节Ca2+内流中的作用。ZP3是透明质酸的糖蛋白激动剂，通过激活具有低选择性阳离子通道特征的百日咳毒素不敏感机制使精子膜去极化。ZP3还激活百日咳毒素敏感的途径，产生短暂的上升，在内部pH值。协同作用的去极化和碱化开放电压敏感的Ca2+通道。这些观察结果表明，哺乳动物精子利用膜电位依赖的信号转导机制，去极化途径是一个上游转导元件耦合粘附受精过程中的分泌。
The sperm acrosome reaction is a Ca(2+)-dependent secretory event required for fertilization. Adhesion to the egg's zona pellucida promotes Ca2+ influx through voltage-sensitive channels, thereby initiating secretion. We used potentiometric fluorescent probes to determine the role of sperm membrane potential in regulating Ca2+ entry. ZP3, the glycoprotein agonist of the zona pellucida, depolarizes sperm membranes by activating a pertussis toxin-insensitive mechanism with the characteristics of a poorly selective cation channel. ZP3 also activates a pertussis toxin-sensitive pathway that produces a transient rise in internal pH. The concerted effects of depolarization and alkalinization open voltage-sensitive Ca2+ channels. These observations suggest that mammalian sperm utilize membrane potential-dependent signal transduction mechanisms and that a depolarization pathway is an upstream transducing element coupling adhesion to secretion during fertilization.