EXPRESSION OF THE CLASS-III BETA-TUBULIN ISOTYPE IN DEVELOPING NEURONS IN CULTURE
EXPRESSION OF THE CLASS-III BETA-TUBULIN ISOTYPE IN DEVELOPING NEURONS IN CULTURE
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DOI：
10.1002/jnr.490320407
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发表时间：
1992-08-01
影响因子：
4.2
通讯作者：
CACERES, A
中科院分区：
文献类型：
--
作者：
FERREIRA, A;CACERES, A
用单抗TuJ1研究了培养的大脑神经元中III型β-微管蛋白亚型的表达。结果表明，在轴突生长的早期，大部分III型β-微管蛋白没有进入微管，这种现象在改变轴突生长反应的速度和程度的情况下也可以观察到。另一方面，在神经元将其轴突分化为轴突和树突后，它与微管的结合显著增加。此外，含有这种异型的秋水仙碱抗性微管的出现与含有高分子量微管相关蛋白(MAP)的稳定微管的出现高度一致。这种模式不同于其他类型的β-微管蛋白在微管中的积聚和掺入。综上所述，我们的结果表明，在发育中的脑神经元中，微管蛋白亚型在体内的利用存在差异，并表明III类β-微管蛋白亚型不是调节早期神经突起生长过程中微管组装的主要因素，但它可能对维持神经突起的进一步延长和/或确定MAP与特定微管亚群的某些独特结合性质很重要。
The expression of the class III beta-tubulin isotype was studied in cultured brain neurons by means of a monoclonal antibody (TuJ1). The results obtained indicate that during early axonal outgrowth most of the class III beta-tubulin is not incorporated into microtubules, a phenomenon which is also observed under conditions which alter the rate and extent of the neurite outgrowth response. On the other hand, a dramatic increase in its incorporation into microtubules is observed after the neurons have differentiated their neurites as axons and dendrites. In addition, the appearance of colchicine-resistant microtubules containing this isotype, a phenomenon which occurs late in neurite development, is highly coincident with the appearance of stable microtubules containing high molecular weight microtubule-associated proteins (MAPs). This pattern is different from that of the accumulation and incorporation of other beta-tubulin isotypes into microtubules. Taken collectively, our results indicate that differences exist in the in vivo utilization of tubulin isotypes in developing brain neurons and suggest that the class III beta-tubulin isotype is not a primary factor involved in the regulation of microtubule assembly during early neurite outgrowth, but that it may be important for maintaining further neurite elongation and/or determining some unique binding property of MAPs to specific microtubule subsets.