Design, Assembly, and Activity of Antisense DNA Nanostructures
Design, Assembly, and Activity of Antisense DNA Nanostructures
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DOI：
10.1002/smll.201101804
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发表时间：
2011-12-16
期刊：
影响因子：
13.3
通讯作者：
Bermudez, Harry
中科院分区：
文献类型：
--
作者：
Keum, Jung-Won;Ahn, Jin-Ho;Bermudez, Harry
离散的DNA纳米结构允许传统DNA形式不可能同时具有的特征：包裹货物，显示多个配体，以及对酶消化的抗性。这些性质表明使用DNA纳米结构作为递送平台。在此，显示反义基序的DNA金字塔显示能够特异性降解mRNA并抑制体外蛋白质表达，并且当与线性DNA相比时，它们显示改善的细胞摄取和基因沉默。此外，这些金字塔的活性可以通过引入适当的互补链来调节。这些结果突出了DNA纳米结构作为功能器件的多功能性。
Discrete DNA nanostructures allow simultaneous features not possible with traditional DNA forms: encapsulation of cargo, display of multiple ligands, and resistance to enzymatic digestion. These properties suggested using DNA nanostructures as a delivery platform. Here, DNA pyramids displaying antisense motifs are shown to be able to specifically degrade mRNA and inhibit protein expression in vitro, and they show improved cell uptake and gene silencing when compared to linear DNA. Furthermore, the activity of these pyramids can be regulated by the introduction of an appropriate complementary strand. These results highlight the versatility of DNA nanostructures as functional devices.