Rapamycin/DiR loaded lipid-polyaniline nanoparticles for dual-modal imaging guided enhanced photothermal and antiangiogenic combination therapy
Rapamycin/DiR loaded lipid-polyaniline nanoparticles for dual-modal imaging guided enhanced photothermal and antiangiogenic combination therapy
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雷帕霉素/DiR负载脂质聚苯胺纳米颗粒用于双模态成像引导增强光热和抗血管生成联合治疗
DOI：
10.1016/j.jconrel.2016.07.005
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发表时间：
2016
影响因子：
10.8
通讯作者：
Li Nan
中科院分区：
文献类型：
--
作者：
Wang Jinping;Guo Fang;Yu Meng;Liu Li;Tan Fengping;Yan Ran;Li Nan
成像引导光热疗法(PTT)在肿瘤治疗中具有广阔的应用前景。然而，到目前为止，成像引导的光热给药系统在肿瘤化学-光热治疗中的成功有限。在这项研究中，作为概念验证，首次报道了一种刺激反应性肿瘤靶向雷帕霉素/DIR载脂聚苯胺纳米粒(RDLPNP)，用于双模式成像引导增强PTT的疗效。在这个体系中，选择了具有有效光热效率和电子共轭体系的聚苯胺(PANI)作为荧光共振能量转移(FRET)的模型受体，负载的菁染料探针(如1，1-dioctadecyl-3，3，3，3-tetramethylindotricarbocyanine碘，DIR)作为近红外荧光的供体。此外，雷帕霉素(RapA)作为抗血管生成的化疗药物，能明显切断肿瘤血管，延缓肿瘤生长。静脉注射RDLPNPs治疗Hela荷瘤小鼠后，随着时间的推移，肿瘤部位出现荧光信号(来自DIR)和增强的光声信号(来自DLPNPs)，并在6h达到高峰。经近红外激光照射后，由于RDLPNPs的光热增强和抗血管生成作用，观察到了良好的抗肿瘤作用。这些结果表明，该多功能纳米颗粒可以作为一种很有前途的成像引导的光热药物输送纳米平台用于癌症治疗。
Imaging-guided photothermal therapy (PTT) has promising application for treating tumors. Nevertheless, so far imaging-guided photothermal drug-delivery systems have been developed with limited success for tumor chemo-photothermal therapy. In this study, as the proof-of-concept, a stimuli-responsive tumor-targeting rapamycin/DiR loaded lipid-polyaniline nanoparticle (RDLPNP) for dual-modal imaging-guided enhanced PTT efficacy is reported for the first time. In this system, polyaniline (PANI) with π-π electronic conjugated system and effective photothermal efficiency is chosen as the appropriate model receptor of fluorescence resonance energy transfer (FRET), and loaded cyanine probe (e.g., 1,1-dioctadecyl-3,3,3,3-tetramethylindotricarbocyanine iodide, DiR) acts as the donor of near-infrared fluorescence (NIRF). In addition, rapamycin (RAPA), which is used as the antiangiogenesis chemotherapeutic drug, can cutdown the tumor vessels and delay tumor growth obviously. After intravenous treatment of RDLPNPs into Hela tumor bearing mice, fluorescent (from DiR) and enhanced photoacoustic (from DLPNPs) signals were found in tumor site over time, which reached to peak at the 6 h time point. After irradiating with an NIR laser, a good anti-tumor effect was observed owing to the enhanced photothermal and antiangiogenic effect of RDLPNPs. These results show that the multifunctional nanoparticle can be used as a promising imaging-guided photothermal drug delivery nanoplatform for cancer therapy.