Regulation of cell adhesion using a signal-responsive membrane substrate.
Regulation of cell adhesion using a signal-responsive membrane substrate.
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DOI：
10.1002/bit.20512
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发表时间：
2005-07
影响因子：
3.8
通讯作者：
Shuhei Okajima;Takeo Yamaguchi;Y. Sakai;S. Nakao
中科院分区：
文献类型：
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作者：
Shuhei Okajima;Takeo Yamaguchi;Y. Sakai;S. Nakao
我们开发了一种新的细胞培养材料，通过改变钾离子浓度来调节细胞粘附。该材料是一种聚乙烯衬底，接枝到热敏聚合物n-异丙基丙烯酰胺（NIPAM）和苯并-18-冠- 6-丙烯酰胺（BCAm）的共聚物上，以垂冠醚作为传感器。冠醚识别钾离子浓度，NIPAM构象变化导致整个聚合物在恒定细胞培养温度下的疏水性/亲水性平衡的变化。虽然细胞在37℃的正常培养基中成功地在离子识别材料上培养，但细胞可以通过单独添加钾离子而不需要蛋白水解酶从材料表面分离，因为细胞附着的表面改变了其表面特性，使其更亲水。因此，成功地恢复了具有完整细胞间连接和高活性的细胞层。此外，通过改变目标传感器，这种材料将能够通过各种细胞信号来控制细胞粘附。
We have developed a novel cell culture material that regulates cell adhesion by changes in potassium ion concentration. The material is a polyethylene substrate grafted to a copolymer of the thermoresponsive polymer N-isopropylacrylamide (NIPAM) and benzo-18-crown- 6-acrylamide (BCAm), with a pendant crown ether as sensor. The crown ether recognizes potassium ion concentrations and NIPAM conformational changes lead to changes in the hydrophobicity/hydrophilicity balance of the entire polymer at constant cell culture temperatures. Although cells were successfully cultured on the ion recognition material in normal culture medium at 37 degrees C, the cells could be detached from the material surface by adding potassium ions alone, without proteolytic enzymes, because the surface to which the cells were attached altered its surface characteristics to a more hydrophilic state. Therefore, cell layers with intact cell-to-cell junctions and high activities were successfully recovered. Furthermore, by changing the target sensors, this material will be able to control cell adhesion through various cellular signals.