Global transcriptomic analysis of Lactobacillus plantarum CAUH2 in response to hydrogen peroxide stress
Global transcriptomic analysis of Lactobacillus plantarum CAUH2 in response to hydrogen peroxide stress
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植物乳杆菌 CAUH2 响应过氧化氢胁迫的整体转录组分析
DOI：
10.1016/j.fm.2019.103389
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发表时间：
2020-05-01
影响因子：
5.3
通讯作者：
Hao, Yanling
中科院分区：
文献类型：
--
作者：
Zhai, Zhengyuan;Yang, Yang;Hao, Yanling
为了克服过氧化氢的有害影响，植物乳杆菌对氧化应激产生适应性反应。在这项研究中，全球转录组学分析表明，L。plantarum CAUH2通过扩大碳源利用谱和促进糖酵解来产生更多的ATP来对抗H2O2胁迫。在转录水平上，NADH过氧化物酶、硫氧还蛋白还原酶和谷胱甘肽过氧化物酶等抗氧化酶对H_2O_2的清除能力分别提高了6.11、36.76和6.23倍。同时，细胞质中游离亚铁离子（Fe2+）维持在较低浓度，限制了芬顿反应，减少了羟自由基的产生。为了修复H_2O_2引起的DNA损伤，L. plantarum CAUH2.此外，蛋氨酸亚砜还原酶和硫氧还蛋白的表达上调，以修复氧化蛋白。值得注意的是，一些转录调控因子（Spx，CcpA和MarR1）被预测参与H2O2胁迫的适应性反应，表明L. plantarum CAUH2利用多种传感器来监测氧化应激并调节H2 O2应激下的转录调节网络。这些发现为L. plantarum以科普氧化应激。
To overcome the deleterious effects of hydrogen peroxide, Lactobacillus plantarum elicits an adaptive response to oxidative stress. In this study, global transcriptomic analysis revealed that L. plantarum CAUH2 expanded its carbon source utilizing profile and enhanced glycolysis to produce more ATP to confront with H2O2 stress. Some antioxidant enzymes including NADH peroxidase, thioredoxin reductase and glutathione peroxidase were 6.11, 36.76 and 6.23-fold up-regulated at transcription level for H2O2 scavenging. Meanwhile, free ferrous iron (Fe2+) was maintained at low concentrations in the cytoplasm, which could limit Fenton reaction and reduce the production of hydroxyl radicals. To repair DNA lesion caused by H2O2, both base excision repair system and recombinational DNA repair pathway were employed by L. plantarum CAUH2. In addition, the expression of methionine sulfoxide reductases and thioredoxin were up-regulated to repair oxidized proteins. It is noteworthy that some transcriptional regulators (Spx, CcpA and MarR1) were predicted to participate in the adaptive response to H2O2 stress, suggesting that L. plantarum CAUH2 utilized a wide array of sensors to monitor oxidative stress and modulated the transcriptional regulation network under H2O2 stress. These findings provide novel insight into the protective mechanisms developed by L. plantarum to cope with oxidative stress.