Distribution of tubulin-containing structures in the egg of the sea urchin Strongylocentrotus purpuratus from fertilization through first cleavage.
Distribution of tubulin-containing structures in the egg of the sea urchin Strongylocentrotus purpuratus from fertilization through first cleavage.
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DOI：
10.1083/jcb.84.3.668
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发表时间：
1980-03
期刊：
影响因子：
--
通讯作者：
Weber K
中科院分区：
文献类型：
--
作者：
Harris P;Osborn M;Weber K
用间接免疫荧光显微镜观察了从受精到第一次卵裂每隔一段时间的海胆卵中含有微管蛋白的结构。染色结果显示，卵母细胞不仅由精子卵母细胞组成，还由源自卵子其他部位的微管蛋白染色纤维组成。怪物不会直接分裂，而是在圆形怪物或间期怪物开始形成之前先被分解。间期紫菀在条纹期达到发育高峰，在有丝分裂器形成之前依次分解。巨噬细胞、间期巨噬细胞以及有丝分裂装置的巨噬细胞从细胞中心或巨噬细胞中心向细胞外围进行分解，然后是新巨噬细胞的生长，也从巨噬细胞中心向外进行。这种模式表明某种条件或因素的瞬态波浪式运动有利于微管解聚。
Eggs of the sea urchin Strongylocentrotus purpuratus were examined by indirect immunofluorescence microscopy for tubulin-containing structures at intervals from fertilization through first cleavage. The staining revealed that the monaster is made up not only of the sperm aster but also of tubulin-staining fibers originating elsewhere in the egg. The monaster does not divide directly but is broken down first before the amphiaster or interphase asters begin to form. The interphase asters reach a peak of development at the streak stage and are in turn broken down before the formation of the mitotic apparatus. The breakdown of the monaster, interphase asters, as well as the asters of the mitotic apparatus proceeds from the cell center or aster centers to the periphery of the cell and is followed by growth of new asters, also proceeding outward from the aster centers. The pattern suggests a transient wavelike movement of some condition, or factor, which favors microtubule depolymerization.