Synergistic protective effect of ischemic preconditioning and allopurinol on ischemia/reperfusion injury in rat liver
Synergistic protective effect of ischemic preconditioning and allopurinol on ischemia/reperfusion injury in rat liver
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DOI：
10.1016/j.bbrc.2006.08.140
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发表时间：
2006-10-27
影响因子：
3.1
通讯作者：
Lee, Sun-Mee
中科院分区：
文献类型：
--
作者：
Lee, Woo-Yong;Lee, Sun-Mee
本研究探讨了缺血预处理（IPC）、别嘌呤醇（Allo）或两者联合应用对肝缺血/再灌注（I/R）所致线粒体损伤程度的影响。I/R使血清转氨酶活性和线粒体脂质过氧化水平升高，而线粒体谷胱甘肽水平降低。IPC或Allo单独使用可减弱这些变化，Alto + IPC具有协同作用。Allo可增加短暂缺血后血清亚硝酸盐和硝酸盐水平。持续缺血再灌注10 min后观察到的显著过氧化物产生被Allo + IPC显著减弱。I/R后分离的线粒体肿胀，Allo + IPC可使其减轻。在缺血结束时，肝脏ATP水平降低，有明显的黄嘌呤积累，这是由Allo + IPC减弱。这些结果表明，IPC和Allo在肝I/R期间协同作用以保护细胞免受线粒体损伤并保护肝脏能量代谢。(c)2006年爱思唯尔公司All rights reserved.
This study examined the effects of ischemic preconditioning (IPC), allopurinol (Allo) or a combination of both on the extent of mitochondrial injury caused by hepatic ischemia/reperfusion (I/R). I/R increased the serum aminotransferase activity and the level of mitochondrial lipid peroxidation, whereas it decreased the mitochondrial glutathione level. Either IPC or Allo alone attenuated these changes with Alto + IPC having a synergistic effect. Allo increased the serum nitrite and nitrate level after brief ischemia. The significant peroxide production observed after 10 min of reperfusion after sustained ischemia was markedly attenuated by Allo + IPC. The mitochondria isolated after I/R were swollen, which was reduced by Allo + IPC. At the end of ischemia, the hepatic ATP level was lower and there was significant xanthine accumulation, which was attenuated by Allo + IPC. These results suggest that IPC and Allo act synergistically to protect cells against mitochondrial injury and preserve the hepatic energy metabolism during hepatic I/R. (c) 2006 Elsevier Inc. All rights reserved.