Manufacture of Concentrated, Lipid-based Oxygen Microbubble Emulsions by High Shear Homogenization and Serial Concentration
Manufacture of Concentrated, Lipid-based Oxygen Microbubble Emulsions by High Shear Homogenization and Serial Concentration
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DOI：
10.3791/51467
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发表时间：
2014-05-01
影响因子：
1.2
通讯作者：
Kheir, John N.
中科院分区：
文献类型：
--
作者：
Thomson, Lindsay M.;Polizzotti, Brian D.;Kheir, John N.
充气微泡已发展成为超声造影剂和药物输送剂。微气泡可以通过使用超声波、机械搅拌、微流体装置或均质处理表面活性剂来产生。最近，基于脂质的氧气微泡（LOMs）被设计用于在医疗紧急情况下静脉输送氧气，逆转危及生命的低氧血症，并防止随后的器官损伤、心脏骤停和死亡。我们提出的方法，大规模生产的高氧微泡使用闭环高剪切均质机。该工艺可在90分钟内产生2 L的浓缩LOMs（体积比90%）。所得气泡的平均直径约为2 μ m，其流变学特征与血液稀释至60%体积时的流变学特征一致。该技术生产的LOMs容量大，氧纯度高，这表明该技术可能对转化研究实验室有用。
Gas-filled microbubbles have been developed as ultrasound contrast and drug delivery agents. Microbubbles can be produced by processing surfactants using sonication, mechanical agitation, microfluidic devices, or homogenization. Recently, lipid-based oxygen microbubbles (LOMs) have been designed to deliver oxygen intravenously during medical emergencies, reversing life-threatening hypoxemia, and preventing subsequent organ injury, cardiac arrest, and death. We present methods for scaled-up production of highly oxygenated microbubbles using a closed-loop high-shear homogenizer. The process can produce 2 L of concentrated LOMs (90% by volume) in 90 min. Resulting bubbles have a mean diameter of similar to 2 mu m, and a rheologic profile consistent with that of blood when diluted to 60 volume %. This technique produces LOMs in high capacity and with high oxygen purity, suggesting that this technique may be useful for translational research labs.