The Mps3 SUN domain is important for chromosome motion and juxtaposition of homologous chromosomes during meiosis
The Mps3 SUN domain is important for chromosome motion and juxtaposition of homologous chromosomes during meiosis
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Mps3 SUN 结构域对于减数分裂过程中染色体运动和同源染色体并置很重要
DOI：
10.1111/j.1365-2443.2011.01554.x
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发表时间：
2011
期刊：
影响因子：
--
通讯作者：
A.Shinohara
中科院分区：
文献类型：
--
作者：
Rao;H.B.D.P.;Shinohara M.;A.Shinohara
在发芽酵母中，Mps3是复制纺锤体极体(SPB)所必需的，在减数分裂过程中对促进染色体运动至关重要。它是SUN(SAD1-UNC-84)结构域蛋白家族的成员，在许多真核生物中定位于内核膜(NE)，在营养生长中优先定位于SPB；在减数分裂前期I，它重新分布到NE内的许多位置。我们构建了一个完全缺乏SUN结构域的突变体Mps 3-SUN。令人惊讶的是，在减数分裂过程中，它们的3个突变不会扰乱SPB的复制或Mps3对NE的定位。然而，在减数分裂前期I，它出现了几个缺陷，包括染色体运动减少，同源染色体之间过早突触，粗线期紧密并列的同源基因减少。这些发现表明，在减数分裂中，Mps3的SUN结构域对于调节染色体运动事件是重要的，这些事件作用于减数分裂染色体并置，进而促进联会复合体的适当形态发生。
In budding yeast, Mps3 is essential for duplicating the spindle pole body (SPB) and is critical for promoting chromosome motion during meiosis. It is a member of the SUN (Sad1‐Unc‐84) domain family of proteins that localizes to the inner nuclear envelope (NE) in many eukaryotic organisms and preferentially localizes to the SPB in vegetative growth; in meiotic prophase I, it redistributes to many sites within the NE. We constructed anmps3mutant,mps3‐sun, which completely lacks the SUN domain. Surprisingly, themps3‐sunmutation does not disrupt SPB duplication or Mps3 localization to the NE in meiosis. However, it confers several defects during meiotic prophase I including reduced chromosome motion, premature synapsis between homologous chromosomes, and reduced levels of closely juxtaposed homologous loci in pachytene. These findings suggest that in meiosis, the Mps3 SUN domain is important for modulating chromosome motion events that act in meiotic chromosome juxtaposition and by extension, promoting proper morphogenesis of the synaptonemal complex.