Imaging second messenger dynamics in developing neural circuits
Imaging second messenger dynamics in developing neural circuits
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DOI：
10.1002/dneu.20619
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发表时间：
2008-05-01
影响因子：
3
通讯作者：
Feller, Maria B.
中科院分区：
文献类型：
--
作者：
Dunn, Timothy A.;Feller, Maria B.
发育中的神经回路的一个特征是它们自发地活跃。有几个例子，包括视网膜、脊髓和海马体，其中自发活动在邻近细胞之间高度相关，大的去极化事件以分钟为周期发生。神经元“解码”这些缓慢振荡的一种可能机制是通过激活第二信使级联，影响转录活性或驱动翻译后修饰。在这里，我们描述了最近的实验，其中成像已被用来表征视网膜神经元中cAMP/PKA第二信使级联的缓慢振荡。我们回顾了这一特定的第二信使级联成像的最新技术，它与细胞内钙浓度变化的密切关系，以及关于它在神经发育中的作用的几种假设。(c) 2008 Wiley期刊有限公司
A characteristic feature of developing neural circuits is that they are spontaneously active. There are several examples, including the retina, spinal cord, and hippocampus, where spontaneous activity is highly correlated among neighboring cells, with large depolarizing events occurring with a periodicity on the order of minutes. One likely mechanism by which neurons can "decode" these slow oscillations is through activation of second messenger cascades that either influence transcriptional activity or drive posttranslational modifications. Here, we describe recent experiments where imaging has been used to characterize slow oscillations in the cAMP/PKA second messenger cascade in retinal neurons. We review the latest techniques in imaging this specific second messenger cascade, its intimate relationship with changes in intracellular calcium concentration, and several hypotheses regarding its role in neurodevelopment. (c) 2008 Wiley Periodicals, Inc.