Role of substance P on histamine H3 antagonist-induced scratching behavior in mice
Role of substance P on histamine H3 antagonist-induced scratching behavior in mice
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DOI：
10.1254/jphs.fpj05028x
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发表时间：
2006-04-01
影响因子：
3.5
通讯作者：
Kamei, C
中科院分区：
文献类型：
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作者：
Hossen, MA;Inoue, T;Kamei, C
本研究的目的是探讨除组胺以外的化学介质在H-3拮抗剂诱导的抓痕行为中的作用。将组胺H-3拮抗剂碘苯普罗pit和氯苯普罗pit （10 nmol/位点）皮下注射于肥大细胞缺陷型（WBB6F1 W/W-v）和野生型（WBB6F1 +/+）小鼠背部吻侧，诱导抓痕行为。随后，测量速激肽NK1拮抗剂spantide的作用60分钟。我们还研究了H-3拮抗剂对大鼠腹膜肥大细胞体外组胺释放的影响。当以0.5 nmol/位点的剂量皮下注射spantide时，明显抑制了反应。此外，碘苯普罗pit和cloben propit (10（-6) - 10(-8) M）不能诱导离体大鼠腹膜肥大细胞释放组胺。我们的研究结果表明，P物质参与了组胺H-3拮抗剂引起的皮肤反应。此外，这些组胺H-3拮抗剂并没有诱导大鼠腹膜肥大细胞释放组胺的显著增加，这表明组胺H-3受体可能不存在于本研究考虑的外周细胞中。
The purpose of the present Study was to investigate the involvement of chemical mediators, other than histamine, in the scratching behavior induced by H-3 antagonists. Scratching behavior was induced by the histamine H-3 antagonists iodophenpropit and clobenpropit (10 nmol/site) when they were injected intradermally into the rostral part of the back of mast-cell-deficient (WBB6F1 W/W-v) and wild-type (WBB6F1 +/+) mice. Subsequently, the effect of spantide, a tachykinin NK1 antagonist, was measured for 60 min. The effects of the H-3 antagonists on in vitro histamine release from rat peritoneal mast cells were also investigated. When spantide was injected intradermally at a dose of 0.5 nmol/site, It significantly inhibited the response. Furthermore, iodophenpropit and cloben\propit (10(-6) - 10(-8) M) did not induce histamine release in isolated rat peritoneal mast cells. Our results indicate that substance P is involved in the skin responses elicited by the histamine H-3 antagonists. Moreover, the fact that these histamine H-3 antagonists did not induce significant increases in the histamine release from rat peritoneal mast cells Suggests that the histamine H-3 receptor may not be present in the peripheral cells considered in this study.