Compromised potassium recycling in the cochlea contributes to conservation of endocochlear potential in a mouse model of age-related hearing loss
Compromised potassium recycling in the cochlea contributes to conservation of endocochlear potential in a mouse model of age-related hearing loss
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DOI：
10.1016/j.neulet.2013.09.028
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发表时间：
2013-10
影响因子：
2.5
通讯作者：
Haidi Yang;H. Xiong;Qiuhong Huang;Jiaqi Pang;Xuqing Zheng;Lian Chen;Rongjun Yu;Yiqing Zheng
中科院分区：
文献类型：
--
作者：
Haidi Yang;H. Xiong;Qiuhong Huang;Jiaqi Pang;Xuqing Zheng;Lian Chen;Rongjun Yu;Yiqing Zheng
C57BL/6株被认为是研究年龄相关性听力损失(AHL)的良好模型。老龄C57BL/6小鼠的特点是严重听力损失，但耳蜗内电位(EP)保持不变。在这里，我们显示了12个月大的C57BL/6小鼠在4、8、16和32 GHz表现出显著的听力损失，而EP保持在正常水平。形态学检查显示，耳蜗底转区外毛细胞明显丢失，血管纹萎缩。荧光免疫组织化学研究显示，钾通道KCNJ10和KCNQ1在SV的表达显著降低。与此相伴随的是，KCNJ10和KCNQ1的mRNA水平也降低。此外，α1-Na、K-ATPase、α2-Na、K-ATPase和NKCC1三种钾转运蛋白也在基因水平上降低了它们的表达。这些观察表明，衰老C57BL/6小鼠的EP的保存归因于SV在相对较低的水平上产生了新的钾内流和外流平衡。
The C57BL/6 strain is considered an excellent model to study age-related hearing loss (AHL). Aging C57BL/6 mice are characterized by profound hearing loss but conservation of the endocochlear potential (EP). Here we show 12-month-old C57BL/6 mice display a notable hearing loss at 4, 8, 16 and 32 kHz while the EP is maintained at normal level. Morphological examination shows significant outer hair cells loss in the cochlear basal turn and atrophy of the stria vascularis (SV). Fluorescence immunohistochemical studies reveal that potassium channel KCNJ10 and KCNQ1 expression dramatically decreased in the SV. Concomitant with this, mRNA levels of KCNJ10 and KCNQ1 are also reduced. In addition, three other potassium transporters, including α1-Na,K-ATPase, α2-Na,K-ATPase and NKCC1, reduce their expression at mRNA levels as well. These observations suggest that conservation of the EP in aging C57BL/6 mice is attributable to the SV generating a new balance for potassium influx and efflux at a relatively lower level.