Expression of endothelial cell IgG Fc receptors and markers on various cultures.
Expression of endothelial cell IgG Fc receptors and markers on various cultures.
复制标题
内皮细胞 IgG Fc 受体和标记物在各种培养物中的表达。
作者：
L. Pan;R. Kreisle;Y. Shi
目标
测定并比较各种培养物中内皮细胞 IgG Fc 受体 (Fc gamma R) 和标记物的表达。
方法
用细胞因子肿瘤坏死因子-α (TNF-α) 和干扰素-γ (IFN-γ) 刺激人乳腺微血管内皮细胞 (HMVEC)、人主动脉内皮细胞 (HAEC)、人脐静脉内皮细胞 (HUVEC) 和犬主动脉内皮细胞 (CAEC)。使用酶联免疫吸附测定 (ELISA) 测量抗 Fc γ 受体 (Fc γ R) I、II 和 III 型抗体的结合。使用抗血管性血友病因子抗体、Dil-低密度脂蛋白（Dil-Ac-LDL）和异硫氰酸荧光素（FITC）标记的欧洲鸢尾凝集素-1检查内皮细胞标志物的组成型表达。
结果
同时用 TNF-α 和 IFN-γ 对所有三种类型的人内皮细胞 (EC) 进行刺激，抗 Fc gamma R II 的结合显着增加，但对犬内皮细胞则不然。当人 EC 在内皮细胞基础培养基 (ECBM) 中培养时，Fc gamma R II 表达增强最为显着。然而，即使在ECBM中培养3代后，CAEC上的FcγR II表达也不能被人类细胞因子诱导。内皮细胞也表现出经典标记物组成型表达的多样性。
结论
这项研究表明，细胞因子 TNF-α 和 IFN-γ 在体外增强人内皮细胞上低亲和力 Fc gamma R 的表达。结果表明，内皮细胞的异质性不仅存在于组成型表达上，还存在于刺激性表达上。
OBJECTIVE
To determine and compare the expression of endothelial cell IgG Fc receptors (Fc gamma R) and markers on various kinds of cultures.
METHODS
Human breast microvascular endothelial cells (HMVEC), human aortic endothelial cells (HAEC), human umbilical vein endothelial cells (HUVEC) and canine aortic endothelial cells (CAEC) were stimulated with cytokines tumor necrosis factor-alpha (TNF-alpha) and interferon-gamma (IFN-gamma). The binding of anti-Fc gamma receptor (Fc gamma R) type I, II and III antibodies was measured using an enzyme-linked immunosorbent assay (ELISA). The constitutive expression of endothelial cell markers was examined using anti-von Willebrand factor antibodies, Dil-low density lipoprotein (Dil-Ac-LDL) and fluorescein isothiocyanate (FITC)-labeled ulex europaeus agglutinin-1.
RESULTS
The binding of anti-Fc gamma R II was significantly increased by the simultaneous stimulation with TNF-alpha and IFN-gamma on all three types of human endothelial cells (ECs), but not on canine endothelial cells. Enhanced Fc gamma R II expression was most significant when human ECs were cultured in endothelial cell basal medium (ECBM). However, the expression of Fc gamma R II on CAECs could not be induced by human cytokines even after they were cultured in ECBM for 3 passages. Endothelial cells also showed diversity for the constitutive expression of classic markers.
CONCLUSIONS
This study demonstrate that cytokines TNF-alpha and IFN-gamma enhance low-affinity Fc gamma R expression on human endothelial cells in vitro. The results indicate that heterogeneity of endothelial cells exists not only on constitutive expression but also on stimulative expression.