Effects of phorbol esters and a diacylglycerol on mouse eggs: inhibition of fertilization and modification of the zona pellucida.
Effects of phorbol esters and a diacylglycerol on mouse eggs: inhibition of fertilization and modification of the zona pellucida.
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佛波酯和二酰基甘油对小鼠卵子的影响：抑制受精和改变透明带。
DOI：
10.1016/0012-1606(87)90221-1
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发表时间：
1987
影响因子：
2.7
通讯作者：
Kopf,GS
中科院分区：
文献类型：
--
作者：
Endo,Y;Schultz,RM;Kopf,GS
用佛波醇酯和二酰基甘油（sn-1，2-二辛酰甘油）处理的小鼠卵子，可抑制精子穿透透明带和受精。这种效应似乎是由透明质酸介导的，因为用这些化合物处理过的无卵受精的程度与未处理的卵相同。此外，精子与在无或有生物活性佛波醇酯存在下孵育的分离的精子的结合是相似的。最后，用佛波醇酯或sn-1，2-二辛酰甘油处理的精子与卵子结合，并经历与未处理精子相同程度的顶体反应。对受精的抑制作用与至少ZP 2的卵诱导的修饰相关，如其在聚丙烯酰胺凝胶中的电泳迁移率的变化所示。此外，处理过的卵透明带的生物学特性发生了变化，精子的结合没有改变，但卵透明带诱导的顶体反应的最后阶段被抑制。从佛波酯或二酰基甘油处理过的卵中获得的卵透明带应该提供一个系统来研究涉及顶体反应诱导的卵透明带蛋白的结构修饰，以及，描绘了包括顶体反应的事件序列。
Sperm penetration of thezona pellucidaand fertilization are inhibited in mouse eggs treated with phorbol esters and the diacylglycerol, sn-1,2-dioctanoyl glycerol. The effect appears mediated by thezona pellucida, sincezona-free eggs treated with these compounds are fertilized to the same extent as untreated eggs. Moreover, the binding of sperm to isolatedzonaeincubated in the absence or presence of biologically active phorbol esters is similar. Last, sperm treated with phorbol esters or sn-1,2-dioctanoyl glycerol bind to eggs and undergo the acrosome reaction to the same extent as untreated sperm. The inhibitory effect on fertilization is correlated with an egg-induced modification of at least ZP2, as manifested by a change in its electrophoretic mobility in polyacrylamide gels. In addition, changes in the biological properties of the treatedzonaeoccur, such that sperm binding is not altered, but that the final stage(s) of thezona-induced acrosome reaction is inhibited.Zonaeobtained from phorbol ester- or diacylglycerol-treated eggs should provide a system to study both the structural modifications of thezonaproteins that are involved in induction of the acrosome reaction, as well as, delineating the sequence of events that comprise the acrosome reaction.