An efficient approach for wholemount preparation of the myenteric plexus of rat colon
An efficient approach for wholemount preparation of the myenteric plexus of rat colon
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DOI：
10.1016/j.jneumeth.2020.109012
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发表时间：
2021-01-15
影响因子：
3
通讯作者：
Tan, Bo
中科院分区：
文献类型：
--
作者：
Huang, Zitong;Liao, Lu;Tan, Bo
背景：肠神经系统（ENS）的神经丛在调节胃肠道功能（例如肌肉收缩活动和营养吸收）中起着至关重要的作用。研究神经丛可以为 ENS 疾病的研究提供重要信息。整体制备是研究神经丛的重要技术。然而，目前可用的方法既耗时又技术含量高。新方法：本研究描述了一种简单快速的方法来制备大鼠结肠纵向肌和肌间神经丛（LMMP）的整体。使用玻璃棒和湿棉签可以轻松地将整体 LMMP 与底层分离。结果：所提出的方法可以轻松分离完整片材中的 LMMP。对整个肠进行免疫荧光组织化学染色，可以清晰地观察肠神经节、神经纤维和肌间神经丛中的几种神经元群亚型。与现有方法比较：与现有的整个肠制备程序相比，所提出的方法可以更快、更有效地从肠段中足量地制备高质量的 LMMP。结论：这项工作提供了一种高效制备肠整体的快速方法。我们的方法可用于原位观察肌间神经丛的形态和功能变化，以进一步研究 ENS。
Background: The nerve plexus of the enteric nervous system (ENS) plays a crucial part in regulating gastrointestinal functions, such as muscle contractile activity and nutrient absorption. Studying the nerve plexus can provide vital information for research on ENS disorders. Whole-mount preparation is an important technique for investigating the nerve plexus. However, currently available methods are time consuming and highly technical.New method: This study describes a simple and rapid method for preparing whole mounts of the longitudinal muscle and myenteric plexuses (LMMPs) of rat colon. Integral LMMPs can be easily separated from the underlying layer by using glass rods and wet cotton swabs.Results: The proposed method allows the easy separation of the LMMPs in intact sheets. Immunofluorescence histochemical staining of whole mounts enable clear visualization of enteric ganglia, nerve fibers, and several subtypes of neuronal populations residing in the myenteric plexus.Comparison with existing methods: Compared with existing procedures for whole-mount preparations, the proposed method achieves a quicker and more efficient preparation of high-quality LMMPs from intestinal segments in sufficient quantity.Conclusions: This work provides a rapid method for efficiently preparing whole mounts of the intestines. Our method can be used for in situ observation of the morphological and functional alterations of the myenteric plexus for further studies on the ENS.