Phospholipidomics reveals differences in glycerophosphoserine profiles of hypothermically stored red blood cells and microvesicles
Phospholipidomics reveals differences in glycerophosphoserine profiles of hypothermically stored red blood cells and microvesicles
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DOI：
10.1016/j.bbamem.2012.10.026
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发表时间：
2013-02-01
影响因子：
3.4
通讯作者：
Acker, Jason P.
中科院分区：
文献类型：
--
作者：
Bicalho, Beatriz;Holovati, Jelena L.;Acker, Jason P.
在其正常的体内生命周期中，红细胞（红细胞，RBC）经历生化变化，导致膜微泡形成和脱落。RBC微泡形成也在血库储存条件下在体外发生，因此微泡（MV）随着储存时间在储存（保存）介质中积累。已经投入了相当大的努力来获得RBC微泡形成过程的机械理解，因为这对于更好地理解疾病和健康中的RBC生物学至关重要。此外，储存的RBC中积累的MV与输血不良炎症反应有关，已知氯仿可提取化合物（因此是亲脂性的）可触发该效应。然而，由于RBC和MV脂质的薄层色谱分辨率始终使人们能够得出高组成相似性的结论，因此尚未广泛进行MV脂质的深入分析。在这里，我们提出了一个轨道阱质谱（MS）的方法来比较磷脂组成的RBC和MV从白细胞减少，低温（2-6摄氏度）存储的RBC单位。我们使用鸟枪质谱分析和电喷雾电离（ESI）源内分离，并证明了高相似性的组成概况，除了甘油磷酸丝氨酸（PS）。PS 38：4和PS 38：1的对比丰度表征了MV和RBC谱，并表明储存相关的微泡形成可能涉及特定膜筏的脱落。这一发现表明，磷脂组学可能有助于更好地了解红细胞微泡形成过程。(C)2012爱思唯尔有限公司版权所有。
During their normal in vivo life cycle erythrocytes (red blood cells, RBCs) undergo biochemical changes leading to membrane microvesiculation and shedding. RBC microvesiculation also occurs in vitro under conditions of blood bank storage, so microvesicles (MVs) accumulate in the storage (preservation) medium over storage time. Considerable effort has been put into gaining a mechanistic understanding of the RBC microvesiculation process, as this is crucial to better understand RBC biology in disease and in health. Additionally, MVs accumulated in stored RBCs have been implicated in transfusion adverse inflammatory reactions, with chloroform extractable compounds, thus lipophilic, known to trigger the effect. However, because thin layer chromatography resolution of RBC and MV lipids has always enabled one to conclude high compositional similarities, in depth analysis of MV lipids has not been extensively pursued. Here we present an orbitrap mass spectrometry (MS) approach to compare the phospholipid composition of RBCs and MVs from leukoreduced, hypothermically (2-6 degrees C) stored RBC units. We used shotgun MS analysis and electrospray ionization (ESI) intra-source separation, and demonstrated high similarity of compositional profiles, except for glycerophosphoserines (PS). Contrasting abundances of PS 38:4 and PS 38:1 characterized MV and RBC profiles and suggested that storage-associated microvesiculation possibly involves shedding of specific membrane rafts. This finding indicates that phospholipidomics could likely contribute to a better understanding of the RBC microvesiculation process. (C) 2012 Elsevier B.V. All rights reserved.