The ORF1 protein encoded by LINE-1: structure and function during L1 retrotransposition.
The ORF1 protein encoded by LINE-1: structure and function during L1 retrotransposition.
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DOI：
10.1155/jbb/2006/45621
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发表时间：
2006
影响因子：
--
通讯作者：
Martin, Sandra L.
中科院分区：
文献类型：
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作者：
Martin, Sandra L.
LINE-1或L1是哺乳动物中自主的非LTR反转录转座子。 反转录转座需要两个L1编码的多肽的功能， ORF 1p和ORF 2p。早期识别预测的 ORF 2的氨基酸序列和已知的核酸内切酶和逆转录酶 酶导致关于ORF 2 p在反转录转座中的功能的可检验的假设。 正如所预测的，ORF 2 p已被证明具有内切酶和反向酶。 转录酶活性。相比之下，没有已知功能的同源物 对我们理解ORF 1 p在反转录转座过程中的功能有很大帮助。然而，尽管如此， 已经取得了重大进展，我们现在知道ORF 1 p是一个高亲和力的 与L1 RNA一起形成核糖核蛋白颗粒的RNA结合蛋白。 此外，ORF 1 p是核酸伴侣，并且该核酸伴侣活性 是L1转位所必需的
LINE-1, or L1 is an autonomous non-LTR retrotransposon in mammals. Retrotransposition requires the function of the two, L1-encoded polypeptides, ORF1p and ORF2p. Early recognition of regions of homology between the predicted amino acid sequence of ORF2 and known endonuclease and reverse transcriptase enzymes led to testable hypotheses regarding the function of ORF2p in retrotransposition. As predicted, ORF2p has been demonstrated to have both endonuclease and reverse transcriptase activities. In contrast, no homologs of known function have contributed to our understanding of the function of ORF1p during retrotransposition. Nevertheless, significant advances have been made such that we now know that ORF1p is a high affinity RNA binding protein that forms a ribonucleoprotein particle together with L1 RNA. Furthermore, ORF1p is a nucleic acid chaperone and this nucleic acid chaperone activity is required for L1 retrotransposition.