MAJOR HISTOCOMPATIBILITY COMPLEX CLASS-I ALLELE-SPECIFIC PEPTIDE LIBRARIES - IDENTIFICATION OF PEPTIDES THAT MIMIC AN H-Y T-CELL EPITOPE
MAJOR HISTOCOMPATIBILITY COMPLEX CLASS-I ALLELE-SPECIFIC PEPTIDE LIBRARIES - IDENTIFICATION OF PEPTIDES THAT MIMIC AN H-Y T-CELL EPITOPE
复制标题
DOI：
10.1002/eji.1830240929
复制
发表时间：
1994-09-01
影响因子：
5.4
通讯作者：
BEVAN, MJ
中科院分区：
文献类型：
--
作者：
GAVIN, MA;DERE, B;BEVAN, MJ
我们描述了一种筛选T细胞抗原随机肽大文库的新方法。构建了两个文库，包含代表H-2K(b)限制性八聚体和H-2D(b)限制性非命名体的主要组织相容性复合体（MHC） I类锚定残基的固定氨基酸。来自kb限制性文库（K(b)L: SXIXFXXL）和D-b限制性文库(D(b)L: XXXXNXXX（M)(I)）的肽分别特异性稳定空K-b和D-b分子。该文库包含模拟几种H-2(b)限制性细胞毒性T淋巴细胞表位的肽，并分离出d -b限制性H-Y表位的21个半模位。通过反相高效液相色谱分析，发现含有鉴定的H-Y序列基序的有限复杂性合成简并肽与天然H-Y表位相似。这种肽也能使雌性小鼠免疫雄性脾细胞。讨论了mhc限制性肽库的几种应用。
We describe a novel method for screening large libraries of random peptides for T cell antigens. Two libraries were constructed, containing fixed amino acids representing the major histocompatibility complex (MHC) class I anchor residues for H-2K(b)-restricted octamers and H-2D(b)-restricted nonamers. Peptides from the Kb-restricted library (K(b)L: SXIXFXXL) and the D-b-restricted library (D(b)L: XXXXNXXX(M)(I)) specifically stabilize empty K-b and D-b molecules, respectively. The libraries contain peptides that mimic several H-2(b)-restricted cytotoxic T lymphocyte epitopes, and 21 mimotopes for a D-b-restricted H-Y epitope were isolated. A degenerate synthetic peptide of limited complexity containing the identified H-Y sequence motif was found to be similar to the natural H-Y epitope by reverse-phase high performance liquid chromatography analysis. This peptide is also capable of immunizing female mice against male splenocytes. Several applications for MHC-restricted peptide libraries are discussed.