Photo-controlled binding of MutS to photo-caged DNA duplexes incorporating 4-O-(2-nitrobenzyl) or 4-O-[2-(2-nitrophenyl)propyl] thymidine
Photo-controlled binding of MutS to photo-caged DNA duplexes incorporating 4-O-(2-nitrobenzyl) or 4-O-[2-(2-nitrophenyl)propyl] thymidine
复制标题
MutS 与包含 4-O-(2-硝基苯甲基) 或 4-O-[2-(2-硝基苯基)丙基]胸苷的光笼 DNA 双链体的光控制结合
DOI：
10.1016/j.bmcl.2016.07.075
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发表时间：
2016
影响因子：
2.7
通讯作者：
Sekine M.
中科院分区：
文献类型：
--
作者：
Seio K;Ohno Y;Ohno K;Takeshita L;Kanamori T;Masaki Y;Sekine M.
错配结合蛋白MutS与DNA双链体中凸起结构的结合受UV照射控制。将4-O-（2-硝基苄基）胸苷或4-O-[2-（2-硝基苯基）丙基]胸苷掺入DNA双链体的凸出位置。MutS不与笼状DNA双链体结合，但在通过光照射去除2-硝基苄基或2-（2-硝基苯基）丙基后结合。通过使用光笼状DNA双链体，我们发现MutS与T7 RNA启动子下游的未笼状DNA的结合微弱地抑制了T7 RNA聚合酶的转录。
Mismatch binding protein MutS binding to bulge structure in DNA duplexes was controlled by UV irradiation. 4-O-(2-Nitrobenzyl)thymidine or 4-O-[2-(2-nitrophenyl)propyl]thymidine was incorporated into DNA duplexes a bulged position. The MutS did not bind to the caged DNA duplexes but bound after removing the 2-nitrobenzyl or 2-(2-nitrophenyl)propyl group by photo-irradiation. By using photo-caged DNA duplex, we revealed that binding of MutS to the uncaged DNA downstream of the T7 RNA promoter weakly inhibited transcription by T7 RNA polymerase.