In vivo evidence for the intermediary role of 3',5'-cyclic AMP in parathyroid hormone-induced stimulation of 1alpha,25-dihydroxyvitamin D3 synthesis in rats.
In vivo evidence for the intermediary role of 3',5'-cyclic AMP in parathyroid hormone-induced stimulation of 1alpha,25-dihydroxyvitamin D3 synthesis in rats.
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3,5-环 AMP 在甲状旁腺激素诱导的大鼠 1α,25-二羟基维生素 D3 合成刺激中起中介作用的体内证据。
DOI：
10.1210/endo-101-3-969
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发表时间：
1977
期刊：
影响因子：
4.8
通讯作者：
E. Ogata
中科院分区：
文献类型：
--
作者：
N. Horiuchi;T. Suda;H. Takahashi;E. Shimazawa;E. Ogata
研究了甲状旁腺激素（PTH）对甲状腺-甲状旁腺切除（TPTX）、维生素d缺乏大鼠肾脏25-羟基维生素D3 (25-OH-D3) 1 α -羟基化的刺激作用。由25-OH-D3产生的1 α，25-二羟基维生素D3 [1 α，25-(OH)2-D3]在血浆中的积累，通过输注cAMP （0.9 MUMOL/H）或DBCAMP （1 MUMOL/H）增强到与PTH （i95- 7.5 U/ H）输注获得的最大值相似的水平。当注入茶碱时，亚最大剂量（1 U/h）的甲状旁腺激素引起类似程度的刺激。从TPTX后18小时开始注射2 μ mol/h的cAMP或7.5 U/h的牛甲状旁腺激素，血浆中1 α，25-(OH)2- d3的积累在6小时内类似地恢复到假手术动物的水平。这些结果有力地支持了cAMP在刺激外源性和内源性PTH诱导的1 α，25-(OH)2-D3生成中起重要中介作用的概念。
Teh stimulatory effect of parathyroid hormone (PTH) on renal 1alpha-hydroxylation of 25-hydroxyvitamin D3 (25-OH-D3) was studied in thyro-parathyroidectomized (TPTX), vitamin D-deficient rats into which bovine PTH, theophylline, cAMP or dibutyryl cAMP (dbcAMP) was constantly infused. The accumulation in plasma of 1alpha,25-dihydroxy-vitamin D3 [1alpha,25-(OH)2-D3], produced from 25-OH-D3, was enhanced by infusion of either cAMP (0.9 MUMOL/H) OR DBCAMP (1 mumol/h) to a level similar to the maximum obtained by PTH (i95--7.5 U/h) infusion. A submaximal dose (1 U/h) of PTH caused a similar extent of stimulation, when infused with theophylline. When either 2 mumol/h of cAMP or 7.5 U/h of bovine PTH was infused starting 18 h after TPTX, the accumulation of 1alpha,25-(OH)2-D3 in plasma was similarly restored withing 6 h to the level found in the sham-operated animals. These results strongly support the concept that cAMP plays an important intermediary role in the stimulation of 1alpha,25-(OH)2-D3 production induced by both exogenous and endogenous PTH.