D-D RECOMBINATION DIVERSIFIES THE CDR-3 REGION OF CHICKEN IMMUNOGLOBULIN HEAVY-CHAINS
D-D RECOMBINATION DIVERSIFIES THE CDR-3 REGION OF CHICKEN IMMUNOGLOBULIN HEAVY-CHAINS
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DOI：
10.1111/j.1365-3083.1991.tb02524.x
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发表时间：
1991-05-01
影响因子：
3.7
通讯作者：
TOIVANEN, P
中科院分区：
文献类型：
--
作者：
MANSIKKA, A;TOIVANEN, P
研究了鸡B细胞胚胎分化过程中D-D重组的发生。 通过聚合酶链式反应从第 12 天法氏囊 cDNA 中扩增 Ig 重 (H) 链，并通过 DNA 测序分析 30 个随机 V-D-J 区域。 在任何 V 区均未观察到基因转换事件，表明在胚胎 B 细胞发育的这一阶段，基因转换引起的 H 链多样化尚未激活。 相比之下，V-D-J 关节区域极其不均匀。 大多数测序的V-D-J接头是通过单种系V-mu-1基因（多种系D元件之一）和单个J基因的直接连接形成的。 然而，三个V-D-J区域的大小明显更长，并且它们的D区域结构表明两个或三个不同种系D元件之间的重组。 因此，目前的数据表明 D-D 重组可能在 Ig H 链库的多样化中发挥作用。
The occurrence of D-D recombination during the embryonic differentiation of chicken B cells was studied. Ig heavy (H) chains were amplified by polymerase chain reaction from day-12 bursal cDNA, and 30 random V-D-J regions were analysed by DNA sequencing. No gene conversion events were observed in any of the V regions, indicating that diversification of the H chains by gene conversion is not yet activated at this stage of embryonic B-cell development. In contrast, the V-D-J joint regions were extremely heterogeneous. Most of the sequenced V-D-J joints were formed by direct joining of the single-germline V-mu-1 gene, one of the multiple-germline D elements, and the single J gene. However, three V-D-J regions were clearly longer in size, and their D-region structure indicated recombination between two or three different germline D elements. Thus, the present data suggest that D-D recombination may have a role in diversification of the Ig H-chain repertoire.