Investigating the effects of size, charge, viscosity and bilayer flexibility on liposomal delivery under convective flow.
Investigating the effects of size, charge, viscosity and bilayer flexibility on liposomal delivery under convective flow.
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DOI：
10.1016/j.ijpharm.2016.08.056
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发表时间：
2016-11
影响因子：
5.8
通讯作者：
M. Narenji;M. Talaee;H. Moghimi
中科院分区：
文献类型：
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作者：
M. Narenji;M. Talaee;H. Moghimi
对流是药物输送（例如渗透泵）中采用的主要传质机制之一，并在体内物质运输（例如血液循环）中起作用。虽然对流流动已被广泛研究，没有太多的数据是可利用的对流行为的纳米粒子，在这里，不同的大小，电荷，双层的灵活性和介质粘度的脂质体遇到对流流动和变化，其性质进行了监测超过48小时。对于大颗粒（>350 nm），中性脂质体（NL）显示出显著的相分离，脂质含量随时间推移而降低，而带正电或带负电的脂质体保持均匀。将NL的尺寸减小至约100 nm解决了相分离问题，但其脂质含量仍显示出减少;在带电的小脂质体中未观察到这样的问题。当大NL的双层柔性增加时，既没有观察到相分离也没有观察到脂质含量降低。将大NL的介质粘度从3.4 cP增加到45.2 cP再次解决了问题，并且观察到均匀的脂质体递送。这些结果表明，大小，电荷，双层的灵活性和粘度影响对流脂质体的交付和均匀的交付是可能的，即使在大脂质体通过优化这些因素。
Convective flow is one of the main mechanisms of mass transfer employed in drug delivery (e.g. osmotic pumps) and working in material transport in the body (e.g. blood circulation). Although convective flow has been investigated extensively, not much data is available on convective behavior of nanoparticles, the subject of the present investigation.Here, liposomes with different sizes, charges, bilayer flexibilities and medium viscosities were encountered convective flow and changes in their properties were monitored over 48 h. For large particles (>350 nm), neutral liposomes (NL) showed significant phase separation and decreased lipid content over time, while positively or negatively charged liposomes remained homogenous. Reduction of size in NLs to about 100 nm resolved phase separation problem, but their lipid content still showed reduction; no such a problem was observed in charged small liposomes. When bilayer flexibility of large NLs was increased, neither phase separation nor decreased lipid content was observed. Increasing medium viscosity for large NLs from 3.4 cP to 45.2 cP again solved the problem and a uniform liposomal delivery was observed. These results indicate that size, charge, bilayer flexibility and viscosity affect convective liposomal delivery and that uniform delivery is possible even in large liposomes by optimizing such factors.