LARGE AND SMALL PROTEOGLYCANS OF OSTEOARTHRITIC AND RHEUMATOID ARTICULAR-CARTILAGE
LARGE AND SMALL PROTEOGLYCANS OF OSTEOARTHRITIC AND RHEUMATOID ARTICULAR-CARTILAGE
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DOI：
10.1002/art.1780380514
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发表时间：
1995-05-01
影响因子：
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通讯作者：
GLANT, TT
中科院分区：
文献类型：
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作者：
CSSZABO, G;ROUGHLEY, PJ;GLANT, TT
Objective.为了鉴定骨关节炎（OA）和类风湿性关节炎（RA）期间关节软骨中大聚集（聚集蛋白聚糖）和小蛋白聚糖（PG）群体的特征性变化。采用琼脂糖-聚丙烯酰胺复合凝胶法分离46例OA和8例RA患者、2例胎儿和6例正常成人股骨髁软骨胍提取物中的聚集蛋白聚糖群，并在12%聚丙烯酰胺凝胶中分析同一提取物中的小PG（双糖聚糖、核心蛋白聚糖和纤维调节蛋白）。用抗聚集蛋白聚糖抗原表位抗体和抗小肽抗体对凝胶进行染色或电泳转移，用放射免疫抑制法比较样品的抗原表位含量。正常和患病的样品之间的电泳迁移率，条带分布，和抗体染色有显着差异。OA和特别是RA样品严重降解，缺乏某些聚集蛋白聚糖群体，并且与正常样品相比含有较少的硫酸角质素和6-硫酸软骨素表位。与正常样品相比，OA样品中4-硫酸软骨素和“胎儿型”表位的水平升高，在患病软骨中发现比正常软骨中更多的小PG的核心蛋白，但在大小和糖胺聚糖取代上更不均一。在患病软骨中存在大的和小的PG的广泛降解，但确实存在修复过程，特别是在OA软骨中，患病软骨的软骨细胞能够合成胎儿型聚集蛋白聚糖。病变软骨中小PG的糖基化与正常软骨不同。
Objective. To identify characteristic changes in large aggregating (aggrecan) and small proteoglycan (PG) populations in articular cartilages during osteoarthritis (OA) and rheumatoid arthritis (RA),Methods. Aggrecan populations in guanidine extracts of femoral condylar cartilages of 46 OA and 8 RA patients who underwent total knee arthroplasty, as well as of 2 fetuses and 6 normal adults, were separated in agarose-polyacrylamide composite gels, Small PGs (biglycan, decorin, and fibromodulin) in the same extracts were analyzed in 12% polyacrylamide gels. Gels were stained or electrophoretically transferred and probed with antibodies to aggrecan epitopes and to small PGs, Epitope contents of the samples were also compared by inhibition radioimmunoassay.Results. There were significant differences found among normal and diseased samples in their electrophoretic mobilities, band distributions, and antibody staining. OA and especially RA samples were heavily degraded, lacked certain aggrecan populations, and contained fewer keratan sulfate and chondroitin-6-sulfate epitopes compared with normal samples, Levels of chondroitin-4-sulfate and ''fetal-type'' epitopes were elevated in the OA samples compared with the normal ones, More core proteins of small PGs were found in diseased than in normal cartilages, but they were more heterogeneous in size and glycosaminoglycan substitution,Conclusion. There is extensive degradation of both large and small PGs in diseased cartilages, but a repair process does exist, especially in OA cartilages, Chondrocytes of diseased cartilages are able to synthesize fetal-type aggrecans. Small PGs are glycosylated differently in diseased cartilages than in normal ones.