Tetrodotoxin poisoning evidenced by solid-phase extraction combining with liquid chromatography-tandem mass spectrometry
Tetrodotoxin poisoning evidenced by solid-phase extraction combining with liquid chromatography-tandem mass spectrometry
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DOI：
10.1016/j.jchromb.2008.06.030
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发表时间：
2008-08-01
影响因子：
3
通讯作者：
Hwang, Deng-Fwu
中科院分区：
文献类型：
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作者：
Jen, Hsiao-Chin;Lin, Shin-Jung;Hwang, Deng-Fwu
对2006年11月发生在台湾高雄的一宗食物麻痹中毒事件中的腹足动物牛油的毒性和毒素成分进行了研究。所得腹足动物消化腺和其他部位的平均毒性评分最高，分别为62 +/- 24 μ g/g（平均+/- sd）和32 +/- 16 μ g/g。从这些腹足类动物中提取的毒素量大，用传统的HPLC-FLD方法容易鉴定为河豚毒素。采用新建立的LC-MS/MS法对患者血清中微量毒素进行分析。LC-MS/MS是由LC系统与MS/MS系统通过涡轮增压喷雾接口连接而成。患者血清TTX采用正离子检测和多反应监测模式。在1 ~ 100 ng/ml浓度范围内呈线性，检出限为0.1 ng/ml。定量限在血清浓度为1 ng/ml时具有重复性。血清中TTX含量为3.30 +/- 0.08 ng/ml。结果表明，LC-MS/MS比以往报道的LC-MS检测TFX的方法可检出性更低，可靠性更高。进一步确定了腹足动物食物中毒的病原体为TTX。(C) 2008 Elsevier B.V.版权所有
The toxicity and toxin component of gastropod Niotha clathrata implicated to a food paralytic poisoning incident in Kaohsiung, Taiwan in November 2006 were studied. The highest scores of average toxicity in the digestive gland and other portions from collected gastropods were 62 +/- 24 (mean +/- S.D.) and 32 +/- 16 mu g/g according to tetroclotoxin (TTX) bioassay, respectively. The toxin from these gastropods was large amount and easily identified as tetroclotoxin by traditional method of HPLC-FLD. The toxin of patient's blood serum was trace amount and analyzed by a new developed method LC-MS/MS. LC-MS/MS was contracted by the LC system interfaced with the MS/MS system with a turbo ion spray interface. Positive ion detection and multiple reaction monitoring mode were used for TTX of patient serum. It was found that linearity in serum was observed within concentration ranged of 1-100 ng/ml and limit of detection was 0.1 ng/ml. The LOQ was reproducible at 1 ng/ml in serum. The blood serum showed to contain TTX of 3.30 +/- 0.08 ng/ml. It indicated that LC-MS/MS was more lower detectable and believable method for TFX determination than LC-MS reported previously. Furthermore, the causative agent of gastropod food poisoning was identified as TTX. (C) 2008 Elsevier B.V. All rights reserved.