Structure and Interaction of Nanoparticle-Protein Complexes
Structure and Interaction of Nanoparticle-Protein Complexes
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DOI：
10.1021/acs.langmuir.8b00110
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发表时间：
2018-05-22
期刊：
影响因子：
3.9
通讯作者：
Kohlbrecher, Joachim
中科院分区：
文献类型：
--
作者：
Kumar, Sugam;Yadav, Indresh;Kohlbrecher, Joachim
纳米粒子与蛋白质的整合具有很高的科学意义，因为它们的复合物显示出惊人的潜力，将纳米粒子的纳米级特性与蛋白质分子的特定结构和功能相结合。纳米颗粒-蛋白质复合物尤其可用于新兴的纳米生物技术领域（纳米医学、药物递送和生物传感器），因为纳米颗粒的尺寸与活细胞相当，可以进入细胞并在细胞内操作。了解纳米颗粒与不同蛋白质分子的相互作用是此类应用的先决条件。两种组分的相互作用已被证明会导致蛋白质的构象变化并影响纳米粒子的表面性质和胶体稳定性。在这篇专题文章中，我们最近的研究探索了纳米粒子-蛋白质系统和所得结构中的驱动相互作用。阴离子胶体二氧化硅纳米颗粒和两种球状带电蛋白质[溶菌酶和牛血清白蛋白（BSA）]已作为模型系统进行了研究。发现两种蛋白质在纳米粒子上的吸附行为完全不同，但它们在各自的纳米粒子-蛋白质系统中都引起相似的从一相到两相的相变。蛋白质的存在分别诱导纳米颗粒与溶菌酶和 BSA 之间的短程和长程吸引力。观察到的相行为及其对各种物理化学参数（例如纳米粒子尺寸、离子强度和溶液 pH 值）的依赖性已通过潜在的相互作用进行了解释。
The integration of nanoparticles with proteins is of high scientific interest due to the amazing potential displayed by their complexes, combining the nanoscale properties of nanoparticles with the specific architectures and functions of the protein molecules. The nanoparticle-protein complexes, in particular, are useful in the emerging field of nanobiotechnology (nanomedicine, drug delivery, and biosensors) as the nanoparticles having sizes comparable to that of living cells can access and operate within the cell. The understanding of nanopartide mteraction with different protein molecules is a prerequisite for such applications. The mteraction of the two components has been shown to result in conformational changes in proteins and to affect the surface properties and colloidal stability of the nanopartides. In this feature article, our recent studies exploring the driving interactions in nanopartide-protein systems and resultant structures are presented The anionic colloidal silica nanoparticles and two globular charged proteins [lysozyme and bovine serum albumin (BSA)] have been investigated as model systems. The adsorption behavior of the two proteins on nanoparticles is found to be completely different, but they both give rise to similar phase transformation from one phase to two phase in respective nanopartide-protein systems. The presence of protein induces the short-range and long-range attraction between the nanoparticles with lysozyme and BSA, respectively. The observed phase behavior and its dependence on vanous physiochemical parameters (e.g, nanopartide size, ionic strength, and solution pH) have been explained in terms of underlying interactions.