A low frequency persistent reservoir of a genomic island in a pathogen population ensures island survival and improves pathogen fitness in a susceptible host.
A low frequency persistent reservoir of a genomic island in a pathogen population ensures island survival and improves pathogen fitness in a susceptible host.
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病原体中基因组岛的低频持续储层可确保岛屿的生存并改善易感宿主的病原体适应性。
DOI：
10.1111/1462-2920.13482
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发表时间：
2016-11
影响因子：
5.1
通讯作者：
Arnold DL
中科院分区：
文献类型：
--
作者：
Neale HC;Laister R;Payne J;Preston G;Jackson RW;Arnold DL
植物病原细菌及其宿主的共同进化是一个复杂的动态过程。宿主抗性对入侵的病原体施加压力，这可以导致细菌基因组的变化，使病原体能够逃避宿主抗性。我们已经观察到这种现象与植物病原体假单胞菌pv。其中已经从其染色体上失去携带效应基因avrPphB的基因组岛PPHGI-1的分离物对先前抗性的植物宿主具有感染性。然而，我们从来没有观察到岛屿灭绝的病原体种群在主机内，这表明岛屿是保持。在这里，我们提出了一个数学模型，该模型预测了岛屿在种群中的不同可能命运;一个结果表明，如果PPHGI-1能够带来健身效益，那么它将在种群中长期保持低频率。我们经验性地测试了这一预测，并确定在宿主抗性期间细菌群体中的PPHGI-1频率下降到低但可持续检测的水平。一旦携带PPHGI-1的细胞遇到易感宿主，它们就会以负频率依赖性方式迅速增加。重要的是，我们的数据表明，移动的遗传元件可以在细菌群体中持续存在，并且在有利条件下频率增加。
The co‐evolution of bacterial plant pathogens and their hosts is a complex and dynamic process. Host resistance imposes stress on invading pathogens that can lead to changes in the bacterial genome enabling the pathogen to escape host resistance. We have observed this phenomenon with the plant pathogen Pseudomonas syringae pv. phaseolicola where isolates that have lost the genomic island PPHGI‐1 carrying the effector gene avrPphB from its chromosome are infective against previously resistant plant hosts. However, we have never observed island extinction from the pathogen population within a host suggesting the island is maintained. Here, we present a mathematical model which predicts different possible fates for the island in the population; one outcome indicated that PPHGI‐1 would be maintained at low frequency in the population long term, if it confers a fitness benefit. We empirically tested this prediction and determined that PPHGI‐1 frequency in the bacterial population drops to a low but consistently detectable level during host resistance. Once PPHGI‐1‐carrying cells encounter a susceptible host, they rapidly increase in the population in a negative frequency‐dependent manner. Importantly, our data show that mobile genetic elements can persist within the bacterial population and increase in frequency under favourable conditions.
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影响因子：
3.3
作者：
Minter EJ;Watts PC;Lowe CD;Brockhurst MA
通讯作者：
Brockhurst MA
影响因子：
6.7
作者：
McCann HC;Rikkerink EH;Bertels F;Fiers M;Lu A;Rees-George J;Andersen MT;Gleave AP;Haubold B;Wohlers MW;Guttman DS;Wang PW;Straub C;Vanneste JL;Rainey PB;Templeton MD
通讯作者：
Templeton MD
影响因子：
6.7
作者：
Godfrey, Scott A. C.;Lovell, Helen C.;Arnold, Dawn L.
通讯作者：
Arnold, Dawn L.
影响因子：
2.1
作者：
Kiss, Tuende;Morgan, Eirwen;Nagy, Gabor
通讯作者：
Nagy, Gabor
影响因子：
2.7
作者：
Taylor, JD;Teverson, DM;Davis, JHC
通讯作者：
Davis, JHC