Structural organization of the human gene encoding nuclear lamin A and nuclear lamin C.
Structural organization of the human gene encoding nuclear lamin A and nuclear lamin C.
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发表时间：
1993-08
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通讯作者：
F. Lin;H. Worman
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作者：
F. Lin;H. Worman
我们已经确定了编码核纤层蛋白A和C（核纤层的中间丝蛋白）的人类基因的结构组织。测序和限制性酶切图谱显示编码区跨越约24个内切酶。5 '-近端启动子区包含几个富含GC的片段、CCAAT盒和序列TATTA的TATA样元件。核纤层蛋白A/C基因包含12个外显子。外显子10内的选择性剪接产生编码前核纤层蛋白A和核纤层蛋白C的两种不同mRNA。因此，产生了两种蛋白质，其中只有一种，前核纤层蛋白A，可以通过异戊二烯化修饰。人核纤层蛋白A/C基因中的内含子位置通常在先前表征的爪蟾核纤层蛋白LIII和小鼠核纤层蛋白B2基因中是保守的，但不同于果蝇核纤层蛋白基因中的那些。在编码中央视杆结构域的区域中，与大多数细胞质中间丝蛋白（除巢蛋白和神经丝蛋白外）基因中的内含子位置相比，内含子位置也是保守的。对这些基因中内含子位置的分析支持核纤层蛋白和其他中间丝蛋白来自共同祖先的假设。
We have determined the structural organization of the human gene that encodes nuclear lamins A and C, intermediate filament proteins of the nuclear lamina. Sequencing and restriction mapping show that the coding region spans approximately 24 kilobases. The 5'-proximal promoter region contains several GC-rich stretches, a CCAAT box, and a TATA-like element of sequence TATTA. The lamin A/C gene contains 12 exons. Alternative splicing within exon 10 gives rise to two different mRNAs that code for pre-lamin A and lamin C. Consequently, two proteins are generated, only one of which, pre-lamin A, can be modified by isoprenylation. The intron positions in the human lamin A/C gene are generally conserved in the previously characterized genes for Xenopus lamin LIII and mouse lamin B2, but different from those in a Drosophila lamin gene. In the regions coding for the central rod domains, the intron positions are also conserved when compared with the intron positions in the genes for most cytoplasmic intermediate filament proteins except those for nestin and neurofilaments. Analysis of the intron positions in these genes supports the hypothesis that the nuclear lamins and other intermediate filament proteins arose from a common ancestor.