Animal model for the study of the relationship between lower urinary tract symptoms/bladder outlet obstruction and erectile dysfunction
Animal model for the study of the relationship between lower urinary tract symptoms/bladder outlet obstruction and erectile dysfunction
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DOI：
10.1111/j.1442-2042.2011.02823.x
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发表时间：
2011-10-01
影响因子：
2.6
通讯作者：
Tsukamoto, Taiji
中科院分区：
文献类型：
--
作者：
Kobayashi, Ko;Kato, Ryuichi;Tsukamoto, Taiji
目的：老年男性的下尿路症状（LUTS）通常与勃起功能障碍（艾德）相关，并且两者都显着影响生活质量。然而，联系LUTS到艾德的机制尚未完全阐明。本研究的目的是建立下尿路综合征（LUTS）/膀胱出口梗阻（BOO）后艾德的动物模型，并探讨该类型艾德发病相关分子的表达。假手术动物作为对照。在梗阻或假手术后4、8和16周评价排尿和勃起功能。阴茎海绵体平滑肌松弛相关基因的mRNA表达通过实时定量逆转录-聚合酶链反应（RT-PCR）进行检测。结果：梗阻后4、8、16周，BOO大鼠排尿和膀胱功能均明显差于假手术组。梗阻后16周BOO大鼠勃起功能明显低于假手术对照组（P < 0.01），而梗阻后4周和8周BOO大鼠勃起功能与假手术组相似。梗阻后16周内皮型一氧化氮合酶（eNOS）mRNA表达较假手术组明显降低（P < 0.01）。结论：建立了一种研究下尿路梗阻与艾德关系的动物模型。内皮型一氧化氮合酶（eNOS）功能受损导致的内皮功能障碍可能参与了下尿路梗阻/膀胱出口梗阻后艾德性ED的发生。
Objectives: Lower urinary tract symptoms (LUTS) are frequently associated with erectile dysfunction (ED) in aged men, and both significantly influence quality of life. However, the mechanism linking LUTS to ED has not been clarified completely. The purpose of the present study was to establish an animal model of ED following LUTS/bladder outlet obstruction (BOO) and investigate the expression of molecules related to the cause of this type of ED.Methods: Male Sprague-Dawley rats with partial BOO were used as an experimental model of LUTS. Sham-operated animals served as controls. Voiding and erectile function were evaluated 4, 8, and 16 weeks after obstruction or sham operation. The mRNA expression of penile tissue genes related to penile corporal smooth muscle relaxation was examined by quantitative real-time reverse transcription-polymerase chain reaction.Results: Voiding and bladder function of BOO rats were significantly worse than in sham-operated rats 4, 8, and 16 weeks after obstruction. The erectile function of BOO rats was significantly decreased compared with that of the sham-operated controls (P < 0.01) 16 weeks after obstruction, although it was similar to that of sham-operated animals at 4 and 8 weeks after obstruction. The expression of endothelial nitric oxide synthase (eNOS) mRNA was significantly decreased 16 weeks after obstruction compared with that in sham-operated rats (P < 0.01).Conclusion: An animal model for investigations into the association between LUTS and ED is described herein. Endothelial dysfunction induced by impaired eNOS function is likely to be involved in ED following LUTS/BOO.