Immunocytochemical techniques reveal multiple, distinct cellular pools of PtdIns4P and PtdIns(4,5)P(2).
Immunocytochemical techniques reveal multiple, distinct cellular pools of PtdIns4P and PtdIns(4,5)P(2).
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DOI：
10.1042/bj20090428
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发表时间：
2009-07-29
期刊：
影响因子：
--
通讯作者：
Irvine RF
中科院分区：
文献类型：
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作者：
Hammond GR;Schiavo G;Irvine RF
PtdIns 4P是合成多功能质膜脂质PtdIns（4，5）P2的主要前体。然而PtdIns 4 P本身也具有调节脂质的功能，特别是在高尔基体处。在本研究中，我们定义了特定的条件，使几个细胞器膜的PtdIns 4P的免疫细胞化学检测保存。我们报告不同的池，这种脂质在高尔基体和质膜，这是由不同的PI 4K（磷脂酰肌醇4-激酶）的活动合成的，也存在PtdIns 4P在细胞质囊泡，这是不容易识别的PI 4K含有贩运中间体。此外，我们提出的证据表明，大多数PtdIns 4P驻留在质膜，在那里它是代谢不同的稳态质膜池PtdIns（4，5）P2。
PtdIns4P is the major precursor for the synthesis of the multifunctional plasma membrane lipid, PtdIns(4,5)P2. Yet PtdIns4P also functions as a regulatory lipid in its own right, particularly at the Golgi apparatus. In the present study we define specific conditions that enable preservation of several organellar membranes for the immunocytochemical detection of PtdIns4P. We report distinct pools of this lipid in both Golgi and plasma membranes, which are synthesized by different PI4K (phosphatidylinositol 4-kinase) activities, and also the presence of PtdIns4P in cytoplasmic vesicles, which are not readily identifiable as PI4K containing trafficking intermediates. In addition, we present evidence that the majority of PtdIns4P resides in the plasma membrane, where it is metabolically distinct from the steady-state plasma membrane pool of PtdIns(4,5)P2.