Growth Inhibition of Bacterial Fish Pathogens and Quorum-Sensing Blocking by Bacteria Recovered from Chilean Salmonid Farms
Growth Inhibition of Bacterial Fish Pathogens and Quorum-Sensing Blocking by Bacteria Recovered from Chilean Salmonid Farms
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DOI：
10.1080/08997659.2014.1001534
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发表时间：
2015-01-01
影响因子：
1.2
通讯作者：
Urrutia, Homero
中科院分区：
文献类型：
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作者：
de la Fuente, Mery;Miranda, Claudio D.;Urrutia, Homero
本研究的主要目的是寻找能够抑制鱼类病原菌嗜水气单胞菌、鳗弧菌和嗜冷黄杆菌生长的细菌菌株，并抑制群体感应系统的阻断。对从智利不同淡水鲑鱼养殖场分离的80株革兰氏阴性菌株进行了研究。我们确定，属于假单胞菌属的10个菌株对至少一种被测试的鱼病原体具有抑制作用。其中，9株能够产生铁载体，2株能够抑制所有被测病原菌的生长。当对80株细菌进行QS阻断活性检测时，只有假单胞菌属的菌株。FF16和R5B1被鉴定为QS阻滞剂。当对QS阻滞剂菌株产生高丝氨酸内酯(HSL)分子的能力进行分析时，薄层分析表明两株菌株都能够产生C6-HSL-和C8-HSL-型分子。菌株R5B1没有表现出生长抑制特性，但菌株FF16对嗜水气单胞菌和嗜冷链霉菌的生长以及铁载体的产生也有抑制作用。假单胞菌属FF16显示出潜在的有用的拮抗特性，并可能成为鲑鱼养殖业的益生菌候选。
The main goal of this study was to find bacterial isolates with the ability to inhibit the growth of the fish pathogens Aeromonas hydrophila, Vibrio anguillarum, and Flavobacterium psychrophilum and to inhibit the blockage of the quorum-sensing (QS) system. A total of 80 gram-negative strains isolated from various freshwater Chilean salmonid farms were studied. We determined that 10 strains belonging to the genus Pseudomonas inhibited at least one of the assayed fish pathogens. Of these, nine strains were able to produce siderophores and two strains were able to inhibit the growth of all assayed pathogenic species. When the 80 strains were examined for QS-blocking activity, only the strains Pseudomonas sp. FF16 and Raoultella planticola R5B1 were identified as QS blockers. When the QS-blocker strains were analyzed for their ability to produce homoserine lactone (HSL) molecules, thin-layer chromatography analysis showed that both strains were able to produce C6-HSL- and C8-HSL-type molecules. Strain R5B1 did not show growth inhibition properties, but strain FF16 also led to inhibition of growth in A. hydrophila and F. psychrophilum as well as to siderophore production. Pseudomonas sp. FF16 exhibited potentially useful antagonistic properties and could be a probiotic candidate for the salmon farming industry.